Interleukin il 6

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Interleukin (IL)-6 is a cytokine that plays a key role in the immune response and inflammation. It is involved in the regulation of various cellular processes, including cell growth, differentiation, and survival.

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IL-6 (324 citations)

4 protocols using interleukin il 6

Flow Cytometric Analysis of Treg Cells

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Foxp3/Transcription Factor Staining Buffer Set, True‐Phos™ Perm Buffer, Cell Staining Buffer, and recombinant human cytokines for in vitro stimulation (IFN‐gamma, interleukin (IL)‐6, IL‐2, IL‐4; all carrier‐free) were purchased from Biolegend.
The following antibodies were used to analyse Tregs via flow cytometry: anti‐CD4 (clone OKT4; APC‐Cy7‐labeled), anti‐CD3 (clone HIT3a; PE‐Cy7) anti‐CD25 (clone M‐A251; PerCP‐labeled), anti‐CD127 (clone A019D5; BV‐421‐labeled), anti‐Foxp3 (clone 206D; AlexaFluor647‐labeled), anti‐CD39 (clone A1; FITC‐labeled), and isotype controls (all from Biolegend). STAT activation was studied using commercially available PE‐labeled antibodies against phospho‐STAT1 (clone A17012A.Rec), phospho‐STAT3 (clone 13A3‐1), phospho‐STAT5 (clone A17016B.Rec), and phospho‐STAT6 (clone A15137E) (all Biolegend).

Dold L., Kalthoff S., Frank L., Zhou T., Esser P., Lutz P., Strassburg C.P., Spengler U, & Langhans B. (2024). STAT activation in regulatory CD4+ T cells of patients with primary sclerosing cholangitis. Immunity, Inflammation and Disease, 12(4), e1248.

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Measuring Inflammatory Markers in BAL Fluid

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Tumor necrosis factor (TNF)-α (BioLegend, CA), Pro-MMP9 (R&D Systems, Abingdon, UK), interleukin(IL)-6 (BioLegend), IL-1β (BioLegend), S100A8/9 (R&D Systems), and HMGB1 (CUSABIO, Houston, TX) levels in bronchoalveolar lavage (BAL) fluid and Der p-specific immunoglobulin E (IgE) level in serum were measured using enzyme-linked immunosorbent assay according to the manufacturer’s instructions. The concentrations of total, oxidized, and reduced glutathione in serum were calculated using a glutathione detection kit (Enzo Life Sciences).

Lee H.S, & Park H.W. (2021). Role of mTOR in the Development of Asthma in Mice With Cigarette Smoke-Induced Cellular Senescence. The Journals of Gerontology Series A: Biological Sciences and Medical Sciences, 77(3), 433-442.

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Retinal Protein and Cytokine Quantification

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Retina tissue were washed and then homogenized in cold PBS. After centrifugation, the supernatant fluids were collected to measure the protein concentrations using a BCA kit (P0012; Beyotime, Nantong, Jiangsu, China). Interleukin (IL)-1β levels in retina and serum were measured by ELISA kit according to the manufacturer’s instruction (ab100704; Abcam, Cambridge, MA, USA). Retina and serum inflammatory cytokines including Tumor Necrosis Factor-alpha (TNFα) (#430907; Biolegend, San Diego, CA, USA) and Interleukin (IL)-6 (#431307; Biolegend) were measured using ELISA kits. Serum lipopolysaccharide (LPS) concentration was determined using a kit (CSB-E13066m; Cusabio, Houston, TX, USA).

Wang X., Yu C., Liu X., Yang J., Feng Y., Wu Y., Xu Y., Zhu Y, & Li W. (2022). Fenofibrate Ameliorated Systemic and Retinal Inflammation and Modulated Gut Microbiota in High-Fat Diet-Induced Mice. Frontiers in Cellular and Infection Microbiology, 12, 839592.

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Quantifying Plasma VWF and Inflammation

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Plasma VWF levels were quantified by enzyme-linked immunosorbent assay (ELISA) on day 10.^{30 (link)} VWF levels in plasma of WT or ADAMTS13^−/− mice were calculated and shown as fold increase over pooled plasma VWF levels of untreated WT or untreated ADAMTS13^−/− mice, respectively. Interleukin (IL)-6 (Biolegend), IL-10 (R&D Systems), soluble P-selectin (sPsel; R&D Systems), and thrombin–antithrom-bin (TAT) complexes (Abcam) were measured by ELISA according to the manufacturer's instructions.

Zitomersky N.L., Demers M., Martinod K., Gallant M., Cifuni S.M., Biswas A., Snapper S, & Wagner D.D. (2017). ADAMTS13 Deficiency Worsens Colitis and Exogenous ADAMTS13 Administration Decreases Colitis Severity in Mice. TH Open: Companion Journal to Thrombosis and Haemostasis, 1(1), e11-e23.

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