Qubit fluorimetric quantitation
The Qubit Fluorimetric Quantitation is a sensitive fluorometer designed to accurately measure the concentration of nucleic acids and proteins in sample solutions. It utilizes fluorescent dyes that selectively bind to the target molecules, providing a quantitative measurement of the sample's concentration.
Lab products found in correlation
8 protocols using qubit fluorimetric quantitation
Gut Microbiome Profiling via 16S rRNA Sequencing
Gut Microbiome Profiling by 16S rRNA
DNA Methylation Profiling Protocol
Genomic DNA Extraction from L. monocytogenes
Whole Genome Sequencing of Gram-positive Bacteria
Library preparation was conducted by using the Nextera XT DNA Sample Kit (Illumina, San Diego, CA, USA). WGS was performed twice a week on a NextSeq 500 platform (Illumina) by using 2 × 150-bp runs. FqCleaner version 3.0 was used to eliminate adaptor sequences (32 (link)), reduce redundant or overrepresented reads (33 (link)), correct sequencing errors (34 (link)), merge overlapping paired reads (35 (link)), and discard reads with Phred scores (measure of the quality of identification of nucleobases generated by automated DNA sequencing)
Wastewater Microbiome and Resistome Profiling
The yield of DNA obtained from the influent water samples ranged from 71 to 244 μg per mL (n = 14) and from 15 to 143 μg per mL (n = 14) for the effluent water samples. To determine the abundances of 7 clinically relevant antibiotic resistant genes including the mcr-1 colistin resistance gene, and the 16S rRNA gene as well as the gene markers for some Enterobacteriaceae qPCR analyses were performed.
Gene Expression Analysis of Cancer Pathways
DC Gene Expression Profiling
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