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4 protocols using lamina c mouse mab

1

Investigating JNK/c-Jun Signaling Pathway

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The following chemical reagents were used in this study: iCRT14 (MedChemExpress, cat# HY16665), sp600125 (Cell Signaling Technology, cat#8177). The following antibodies were used in this study: phospho(p)-c-Jun (Ser73) rabbit monoclonal antibody (Cell Signaling Technology, cat# 3270), c-Jun rabbit mAb (Cell Signaling Technology, cat# 9165), p-JNK (Thr183/Tyr185) rabbit mAb (Cell Signaling Technology, cat# 9251), JNK rabbit polyclonal antibody (pAb) (Cell Signaling Technology, cat# 9252). p-β-catenin (Ser552) rabbit mAb (Cell Signaling Technology, cat# 9566). β-catenin (Ser552) rabbit mAb (Abcam, cat# ab32572), mouse control IgG (ABclonal, cat# AC011), rabbit control IgG (ABclonal, cat# AC005), laminA/C mouse mAb (Santa Cruz Biotechnology, cat# sc-376248), β-Tubulin rabbit pAb (Abclonal, cat# AC015), GAPDH mouse mAb (Cell Signaling Technology, cat# 2118), β-actin rabbit mAb (Cell Signaling Technology, cat# 4970), Alexa Fluor 488®-conjugated goat anti-rabbit IgG (H + L) (Invitrogen, cat# A-11008), HRP- (horseradish peroxidase-) conjugated goat anti-mouse IgG (Cell Signaling Technology, cat# 7076), and goat anti-rabbit IgG (Cell Signaling Technology, cat# 7074). Goat anti-BoHV-1 serum was purchased from VMDR Inc (cat# 20PAB-IBR).
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2

Western Blot Antibody Validation

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The following antibodies were used in this study: CPT1A rabbit polyclonal antibody (pAb)(cat# A5307), β-Actin rabbit mAb (cat# AC026), and β-Tubulin Rabbit pAb(cat# AC015) were bought from Abclonal Technology (Woburn, MA, USA). COXIV rabbit pAb(cat#4844 S), HRP conjugated goat anti-mouse IgG (cat# 7076), and HRP labeled goat anti-rabbit IgG (cat# 7074) were purchased from Cell Signaling Technology (Danvers, MA, USA). LaminA/C mouse mAb (cat# sc-376248) was provided by Santa Cruz Biotechnology (Dallas, TX, USA). BoHV-1 gD mAb (cat#1B8-F11), BoHV-1 gC mAb (cat#F2), and goat anti-BoHV-1 serum (cat# PAB-IBR) were provided by VMRD Inc. (Pullman, WA, USA). Donkey anti-goat IgG H&L (HRP) (ca#ab97110) was supplied by Abcam (Cambridge, UK). Alexa Fluor 488®-conjugated goat anti-rabbit IgG (H + L) (cat# A-11008) was provided by Invitrogen Life Technologies (Waltham, MA, USA). CPT1A-specific inhibitor Etomoxir (cat#HY-50202) was ordered from MedChemExpress (Monmouth Junction, NJ, USA).
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3

Molecular Mechanisms of HMGB1-RAGE Signaling

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iCRT14 (MedChemExpress, cat# HY16665), Glycyrrhizin acid (MedChemExpress, cat# HY-N0184). HMGB1 rabbit monoclonal antibody (mAb) (ABclonal, cat# A19529), RAGE rabbit polyclonal antibody (pAb) (ABclonal, cat# A1395), β-catenin rabbit mAb (Abcam, cat# ab32572), laminA/C mouse mAb (Santa Cruz Biotechnology, cat# sc-376,248), Cox IV rabbit pAb (Cell Signaling Technology, cat# 4844), β-Tubulin rabbit pAb (Abclonal, cat# AC015), GAPDH mouse mAb (Cell Signaling Technology, cat# 2118), BoHV-1 gC mouse mAb (VMDR Inc, cat# F2), BoHV-1 gD mouse mAb (VMDR Inc, cat# 1B8-F11), Alexa Fluor 488®-conjugated goat anti-rabbit IgG (H + L) (Invitrogen, cat# A-11,008), HRP- (horseradish peroxidase-) conjugated goat anti-mouse IgG (Cell Signaling Technology, cat# 7076), and goat anti-rabbit IgG (Cell Signaling Technology, cat# 7074).
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4

Antibody Panel for BoHV-1 Research

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The following antibodies were used in this study: NFAT5 monoclonal antibody (MAb) (cat# sc-398171) and LaminA/C mouse MAb (cat# sc-376248) were provided by Santa Cruz Biotechnology (Dallas, TX, USA). HSP60 rabbit polyclonal antibody (pAb) (cat#A0969) and β-Tubulin rabbit pAb (cat# AC015) were obtained from Abclonal (Woburn, MA, USA). β-actin rabbit MAb (cat# 4970), HRP conjugated goat anti-mouse IgG (cat# 7076), as well as HRP, labeled goat anti-rabbit IgG (cat# 7074) were purchased from Cell Signaling Technology (Danvers, MA, USA). BoHV-1 gC MAb (cat#F2) was provided by VMRD Inc. (Pullman, WA, USA). Alexa Fluor 633-conjugated goat pAb to mouse IgG (cat#A-21052) was provided by Invitrogen Life Technologies (Waltham, MA, USA).
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