All participants underwent a fasting blood draw at baseline and week 52. Serum and EDTA-preserved plasma were processed and stored at −80°C. Ultra-high-sensitivity estradiol (Diagnostic System Laboratories, Webster, TX) and testosterone (MP Biomedicals, Irvine, CA) were quantified using a radioimmunoassay (Perkin Elmer 2470 Automatic Gamma Counter). SHBG (Alpco, Salem, NH) was quantified using an enzyme-linked immunosorbent assay (BioTek EPOCH Microplate Spectrophotometer). Baseline and week 52 samples were assayed simultaneously and in duplicate at the end of the study. Intra- and inter-assay coefficients of variation for all samples were ≤10%.
Testosterone
Manufactured by MP Biomedicals
Testosterone is a laboratory analytical standard used for the identification and quantification of testosterone in various sample matrices. It is a naturally occurring steroid hormone that plays a crucial role in the development and maintenance of male sexual characteristics. This product is intended for research and analytical use only, and its description is limited to its core function as a reference standard.
Automatically generated - may contain errors
3 protocols using testosterone
1
Hormonal Biomarkers in Fasting Samples
2
Hormonal and Metabolic Profiling in Rats
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Serum rat leptin, rat insulin, testosterone, free T4, total T3 and glucose were measured (assay range, intra-assay CV%) with the following commercial RIA kits: rat leptin (0.801–200 ng/ml, 3.2) and rat insulin (0.0329–2.0 ng/ml, 4.8) (Linco Research, Inc., St. Charles, MO), testosterone (0.05–40 ng/ml, 6.7), free T4 (0.045–60 ng/DL, 4.6) and total T3 (0.06–80 pg/ml, 4.8) (MP Biomedical, Solon, OH). Serum glucose was measured with the Contour Next EZ Blood Glucose Monitoring System (Ascensia Diabetes Care US, Inc., Parsippany, NJ).
3
Serum Hormone and Glucose Measurement
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Serum rat leptin, rat insulin, testosterone, free T4¸ total T3 and glucose were measured (assay range, intra‐assay CV%) with the following commercial RIA kits: rat leptin (0.801–200 ng/ml, 3.2) and rat insulin (0.0329–2.0 ng/ml, 4.8) (Linco Research, Inc.), testosterone (0.05–40 ng/ml, 6.7), free T4 (0.045–60 ng/dl, 4.6) and total T3 (0.06–80 pg/ml, 4.8) (MP Biomedical). Serum glucose was measured with the Contour Next EZ Blood Glucose Monitoring System (Ascensia Diabetes Care US, Inc.).
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