Iodide

Manufactured by Merck Group

Sourced in United States

Iodide is a chemical compound that consists of iodine and a metal or ammonium ion. It is commonly used in various laboratory applications as a reagent or intermediate. Iodide serves as a source of iodide ions, which can be used in various chemical reactions and analytical procedures. The specific details and intended use of iodide may vary depending on the application and the requirements of the laboratory.

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Lab products found in correlation

8 protocols using iodide

Synthesis of Conjugated Polymer Electrodes

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All chemical reagents were
used as received without further purification. Toluene (anhydrous,
99.8%), borane–tetrahydrofuran complex solution (BH₃OC₄H₈, 1.0 M in THF), 1,4-diethynylbenzene
(C₁₀H₆, 96%), 1-octene (C₁₀H₁₆, 98%), bis(triphenylphosphine)palladium(II) dichloride (Pd-(PPh₃)₂Cl₂, 98%), copper(I) iodide (CuI,
>98.0%), triethylamine (TEA) (N(C₂H₅)₃, 99.0%), vinylene carbonate (VC) (99%), sodium chloride (NaCl)
(≥98.0%),
and poly(acrylic acid) (PAA) (average M_v ∼ 450 000) were purchased from Sigma-Aldrich. Hydrofluoric
acid (HF, 48–51%) and ammonium hydroxide solution (NH₄OH, 25%) were purchased from J.T. Baker and Acros Organic, respectively.
Super P carbon black, n-methyl-2-pyrrolidone (NMP),
Cu-foil, and Li-metal were obtained from Wellcos Corporation (South
Korea). Ethanol (EtOH) (C₂H₅OH, 99.5%) and methanol
(MeOH) (CH₃OH, 99.5%) were purchased from Dae-Jung (South
Korea). 1-Bromo-4-ethynylbenzene (C₈H₅Br, >98.0%)
was purchased from TCI. One molar lithium hexafluorophosphate (LiPF₆) in ethylene carbonate (EC)/ethyl methyl carbonate (EMC)
(1:1 v/v) was obtained from Soul-Brain (South Korea).

Choi Y.H., Park H., Lee S, & Jeong H.D. (2020). Synthesis and Electrochemical Performance of π-Conjugated Molecule Bridged Silicon Quantum Dot Cluster as Anode Material for Lithium-Ion Batteries. ACS Omega, 5(15), 8629-8637.

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Iodide and Tellurium Quantification

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Stock solutions of 1000 mg/L iodide (as potassium iodide) and 1000 mg/L Te (tellurium) were purchased from Sigma-Aldrich (Saint Louis, USA), and TMAH (99.9999%) at 25% w/v from VWR (Radnor, USA). Intermediate solutions of 1 mg/L iodide in 0.5% TMAH and 10 mg/L Te in 0.5% TMAH were prepared through dilution of the stock solutions with double-distilled water. Calibration blanks and final calibration standards of 0.5, 1, 2, and 5 μg/L iodide were prepared after additional dilution of the intermediate solution with double-distilled water and addition of Te to a concentration of 500 μg/L (as an internal standard for ICP-MS measurements). The multi-elemental Elan DRC II smart tuning solution (Perkin Elmer, Massachusetts, USA) was used for daily tuning of the ICP-MS.

Neven K.Y., Marien C.B., Janssen B.G., Roels H.A., Waegeneers N., Nawrot T.S, & Ruttens A. (2020). Variability of iodine concentrations in the human placenta. Scientific Reports, 10, 161.

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ANGPTL4 Modulates BMSC ROS and Apoptosis

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A certain dose of ANGPTL4 was used to treat 1 × 10⁵ BMSCs, cultured in 12 well plate with serum‐free DMEM for 24 h, centrifuged at 1500 g for 5 min, and washed twice with 1 × PBS. The cells were fixed with 70% ethanol (Solarbio, Beijing), placed at 20°C for 15 min, centrifuged at 1500 g for 5 min, and washed twice by precooling 1 × PBS. Subsequently, the cells were incubated with 10 μg/μl Dnase‐free RnaseA (Sigma, St. Louis, MO, USA) to remove RNA, and washed twice with precooled 1 × PBS. Finally, after centrifugation at 150 g for 5 min, the cells were incubated with 1 mg/ml iodide (Sigma) in the dark at 4°C for 12 min. Flow cytometry and ModiFit software (Olympus, Tokyo, Japan) were used to quantify the reactive oxygen species (ROS) level and glucose uptake of BMSCs. In addition, according to the instructions, annexin FITC/PI apoptosis detection kit (Beyotime, Nanjing, China) was combined with flow cytometry to detect apoptosis (Dong & Cui, 2017 ). ModiFit software (Olympus, Tokyo, Japan) was performed to analyze the data.

Zhang F., Wu J., Li X., Ying X., Fang W, & Dong Y. (2022). Angiopoietin‐like protein 4 treated bone marrow‐derived mesenchymal stem cells alleviate myocardial injury of patients with myocardial infarction. Nursing & Health Sciences, 24(1), 312-321.

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Cell Cycle and Apoptosis Analysis

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A total of 1×10⁵ SW480 or LoVo cells were cultured in 12-well plate by using serum-free DMEM for 24 h, centrifuged at 150 × g for 5 min, and washed twice with pre-cooled 1X PBS. Subsequently, the cells were fixed with 70% ethanol (Solarbio), maintained at 20°C for 15 min, centrifuged at 150 × g for 5 min, and washed twice with pre-chilled 1X PBS. Next, the cells were incubated with 10 µg/µl DNase-free RNaseA (Sigma-Aldrich; Merck KGaA) for 45 min at 37°C to eliminate RNA, and washed twice with pre-chilled 1X PBS. Finally, following centrifuging at 150 × g for 5 min, the cells were incubated with 1 mg/ml iodide (Sigma-Aldrich; Merck KGaA) in the dark at 4°C for 12 min. The cell distribution at each phase of the cell cycle was quantified in a flow cytometer and using ModFit software 3.3 (Verity Software House). In addition, according to the manufacturer's instructions, Annexin-FITC/PI Apoptosis Detection Kit (Beyotime Institute of Biotechnology) was combined with flow cytometry to detect cell apoptosis (19 (link)). ModFit software 3.3 was performed to analyze the data.

Qian J., Garg A., Li F., Shen Q, & Xiao K. (2020). lncRNA LUNAR1 accelerates colorectal cancer progression by targeting the miR-495-3p/MYCBP axis. International Journal of Oncology, 57(5), 1157-1168.

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Cell Cycle Analysis of Trophoblast Cells

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After being cultivated in 12-well plates with 1 × 10⁵ cellular structures/well JEG-3 and JAR cellular structures per well for 24 hours in serum-free RPMI-1640 media, the cellular structures were shaken for 5 min at 150 g at a speed of g. then twice rinsed with precooled 1PBS. The cellular structures were then preserved in 70% ethanol (CEF 590056, Solarbio, Peking, China) for 15 minutes at 20 degrees, centrifugation at 150 g for 5 minutes, and washed twice with chilled 1PBS. To eliminate residual RNA, cellular structures were treated for 45 minutes at 37 degrees with 10 g/L DNase-free RNase A (Sigma, Saint Louis, US). After two washes with ice-cold 1PBS, the cellular structures were centrifuged at 150 g for 5 minutes and then treated with 1 mg/mL iodide (Sigma, Saint Louis, US) for 12 minutes at 4°C in the dark. This action was taken to remove any residual RNA. Olympus, Tokyo, Japan, used FACS analysis to evaluate the distribution of cellular structures at each phase of the cell cycle and to identify cell apoptosis using an Annexin-FITC/PI apoptosis Identification Kit (Beyotime, Nanjing, China). All FACS data were processed and analyzed with ModiFit software (Olympus, Tokyo, Japan).

Li Q., Wang Y., Liu F., Wang H, & Fan Y. (2022). LRSAM1 E3 Ubiquitin Ligase Promotes Choriocarcinoma Progression and Metastasis via p53/p21 Signaling Impediment. BioMed Research International, 2022, 1926605.

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Spectrophotometric Analysis of Anionic Salts

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Rosmarinic acid, sodium borohydride, ascorbic
acid, HEPES buffer, sodium phosphate monobasic, and agarose (low melting
point) were purchased from Sigma-Aldrich. AgNO₃ was purchased
from S. D. Fine Chemicals. Tetrabutylammonium (TBA⁺) salts
of fluoride, bromide, chloride, iodide, periodate, cyanide, dihydrogen
phosphate, acetate, nitrate, and bisulfate were purchased from Sigma
Aldrich. Potassium dichromate was purchased from Merck Ltd. Solutions
of all the chemicals were prepared in Milli-Q water.

Bhatt S., Vyas G, & Paul P. (2022). Rosmarinic Acid-Capped Silver Nanoparticles for Colorimetric Detection of CN– and Redox-Modulated Surface Reaction-Aided Detection of Cr(VI) in Water. ACS Omega, 7(1), 1318-1328.

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Cell Cycle Analysis and Apoptosis Assay

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A total of 1 × 10⁵ chondrocytes were cultured in a 12-well plate using serum-free DMEM for 24 h, centrifuged at 1500 g for 5 min and washed with pre-cooled 1 × PBS twice. Subsequently, cells were fixed with 70% ethanol (Solarbio, Beijing, China), placed at 20 °C for 15 min, then centrifuged at 1500 g for 5 min, and washed twice with pre-chilled 1 × PBS. Subsequently, cells were incubated with 10 μg/μL DNase-free RNaseA (Sigma, St. Louis, USA) for 45 min at 37 °C to eliminate RNA, and washed twice with pre-chilled 1 × PBS. Finally, following centrifuging at 150g for 5 min, the cells were incubated with 1 mg/mL iodide (Sigma, St. Louis, USA) in the dark at 4 °C for 12 min. The percentage of cells at each cell cycle phase is quantified in a flow cytometer and analyzed by ModiFit software (Olympus, Tokyo, Japan). Besides, according to instructions, Annexin-FITC/PI Apoptosis Detection Kit (Beyotime, Nanjing, China) was combined with flow cytometry to detect cell apoptosis, according to manufacturer’s instructions [17 (link)]. In addition, modified software (Olympus, Tokyo, Japan) was performed to analyze the data.

Wang C., Wang L., Guan X, & Yue C. (2021). MiR-4303 relieves chondrocyte inflammation by targeting ASPN in osteoarthritis. Journal of Orthopaedic Surgery and Research, 16, 618.

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SV40 MES13 Cell Cycle Analysis

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Cell cycles of SV40 MES13 cells were detected by flow cytometry. In detail, 1 × 10 5 SV40 MES13 cells were cultured in 12-well plates with serum-free DMEM for 24 h, centrifuged at 1,500 g for 5 min, and washed twice with pre-cooled 1 × PBS. The cells were then fixed with 70% ethanol (Solarbio), placed for 15 min, centrifuged at 1,500 g for 5 min, and washed twice with pre-cooled 1 × PBS. Cell distribution at each stage of the cell cycle was quantified in flow cytometers and Mod-iFit software (Olympus, Tokyo, Japan) after incubating for 12 min in the dark at 4°C with 1 mg/mL iodide (Sigma, St. Louis, MO, USA).

Chen Y.X., Zhu S.Y., Huang C., Xu C.Y., Fang X.D, & Tu W.P. (2022). LncRNA Dlx6os1 Accelerates Diabetic Nephropathy Progression by Epigenetically Repressing SOX6 via Recruiting EZH2. Kidney & blood pressure research, 47(3).

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