1-Bromohexane (Aldrich, 98%), 2-(dimethylamino)ethyl methacrylate (DMAEMA) (Aldrich, 98%), oligo (ethylene glycol) dimethacrylate (OEG-DMA) Mn 550 g mol−1 (Aldrich, 98%), ammonium persulfate (APS) (Sigma-Aldrich, 98%), N,N,N′,N″,N″-pentamethyldiethylenetriamine (PMDETA) (Aldrich, 99%), chloroform (CHCl3) (VWR Chemicals), acetonitrile (ACN) (VWR Chemicals) and diethyl ether (DEE) (Merck) were used as received. Milli-Q water with a resistivity of >18 MΩ⋅cm was obtained from an in-line Millipore RiOs/Origin water purification system.
Rios origin water purification system
Manufactured by Merck Group
The RiOs/Origin water purification system is a laboratory equipment product that provides purified water for various applications. It utilizes a combination of filtration and deionization technologies to remove impurities and produce high-quality water. The core function of this system is to supply purified water with consistent quality and purity levels.
Automatically generated - may contain errors
Lab products found in correlation
Cerium 3 nitrate hexahydrate, by Merck Group (2 mentions)
2 methylimidazole, by Merck Group (2 mentions)
Tris 2 2 bipyridine ruthenium chloride, by Merck Group (2 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
1 bromohexane, by Merck Group (1 mentions)
Chloroform chcl3, by Avantor (1 mentions)
Ammonium persulfate ap, by Merck Group (1 mentions)
Acetonitrile acn, by Avantor (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Alendronate sodium, by Merck Group (1 mentions)
Rhodamine b, by Merck Group (1 mentions)
Riboflavin, by Merck Group (1 mentions)
Bca protein concentration determination kit, by Beyotime (1 mentions)
Reactive oxygen detection kit, by Beyotime (1 mentions)
Ethylenediamine, by Merck Group (1 mentions)
Cck 8 kit, by Beyotime (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
Dicyandiamide, by Maclin Biochemical Technology (1 mentions)
5 protocols using rios origin water purification system
1
Synthesis of Functional Polymeric Materials
2
Cerium-Based Multifunctional Nanoplatform
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Cerium(III) nitrate hexahydrate (Ce(NO3)3·6H2O), alendronate sodium (AL), Tris(2,2-bipyridine) ruthenium chloride (RDPP), 2-methylimidazole (HMIM), riboflavin, rhodamine B, H2O2 (technical grade, 30%), LPS, and ORO were provided by Sigma–Aldrich (USA). Zoledronic acid hydrate (ZOL) was obtained from Damas-beta. PB and nitrotetrazolium blue chloride (NBT) were purchased from TCI (Japan). The CCK-8 kit was purchased from MCE (USA). oxLDL was purchased from Yiyuan Biotech (China). Chlorin e6 (Ce6) was provided by Frontier Scientific. A BCA protein concentration determination kit and a reactive oxygen detection kit were obtained from Beyotime Biotechnology. Penicillin–streptomycin solution (100X), fetal bovine serum (FBS), trypsin, Dulbecco’s modified Eagle’s medium (DMEM) and RPMI 1640 medium (1640) were obtained from Gibco (USA). High-purity (Milli-Q) water with a resistivity of 18.2 MΩ cm was obtained from an inline Millipore RiOs/Origin water purification system.
3
Nanoparticle Synthesis and Characterization
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N‐succinimidyl methacrylate, AIBN, and dicyandiamide were obtained from Macklin (China). Ethylenediamine was purchased from Sigma‐Aldrich (USA), and bis(thiobenzoyl) disulfide (CPADN) was purchased from TCI (Japan). MeO‐PEG (mPEG) was obtained from RuixiBio (Beijing, China). The CCK8 kit was purchased from Beyotime (China). GFP mRNA, T7E1 endonuclease, and RNA Synthesis, and Purification kits were purchased from New England Biolabs (USA). Cas9‐mRNA was purchased from TriLink (USA). Antibodies against PCSK9 and LDLR were purchased from Abcam (Cambridge, UK). Lipofectamine 3000 was purchased from Thermo Fisher Scientific (USA). Penicillin–streptomycin solution (100×), trypsin, fetal bovine serum (FBS), and Dulbecco's modified Eagle medium (DMEM) were purchased from Gibco (USA). High‐purity (Milli‐Q) water with a resistivity of 18.2 MΩ cm was obtained from an inline Millipore RiOs/Origin water purification system.
4
Multifunctional Nanoparticle Synthesis and Applications
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Cerium (III) nitrate hexahydrate (Ce (NO3)3·6H2O), glucose (Glu), curcumin, alendronate (AL), Tris (2,2-bipyridine) ruthenium chloride (Ru(ddp)), 2-methylimidazole (HMIM), IR780, IR783, IR808, Ce6, Cy3, copper nitrate trihydrate (Cu(NO3)2·3H2O), manganese dichloride (MnCl2), magnesium chloride (MgCl2), cobalt chloride (CoCl2), iron trichloride (FeCl3) and streptozotocin (STZ) were provided by Sigma–Aldrich (USA). Chlorin e6 (Ce6) was obtained from Frontier (UK). glucose oxidase (GOx) and nitrotetrazolium blue chloride (NBT) were purchased from TCI (Japan). Methyl red, 2′,7′-dichlorofluorescin diacetate (DCFH-DA), hematoxylin-eosin (H&E), Masson's trichrome, Sirius staining solution and dihydroethidium (DHE) were purchased from Beyotime Biotechnology (China). Penicillin–streptomycin solution (100X), fetal bovine serum (FBS), trypsin and Dulbecco's modified Eagle's medium (DMEM) and RPMI-1640 medium (1640) were obtained from Gibco (USA). The Cell Counting Kit-8(CCK-8 kit) was purchased from MCE (USA). Anti-CD31 antibody, anti-α-SMA antibody and goat anti-rabbit IgG secondary antibody were purchased from Abcam (UK). High-purity (Milli-Q) water with a resistivity of 18.2 MΩ cm was obtained from an inline Millipore RiOs/Origin water purification system.
5
Detailed Reagents and Materials Protocol
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Manganese(II) chloride tetrahydrate (MnCl2•4H2O), tris-(hydroxymethyl)aminomethane (TRIS), ethanol, tetrahydrofuran (THF), thiazolyl blue tetrazolium bromide (MTT), cyclohexane, Triton X-100, 1-hexanol, and radioimmunoprecipitation (RIPA) lysis buffer were purchased from Sigma-Aldrich and used as received without further purification. 8-Arm-poly(ethylene glycol) succinimidyl succinate (8-arm-PEG-NHS, hexaglycerol core, molecular weight 10 kDa) was purchased from JenKem Technology (USA). Multi-element ICP standard solutions and nitric acid (ultrapure) for inductively coupled plasma-optical emission spectroscopy (ICP-OES) analysis were purchased from Merck KGaA (Germany). Dulbecco's Modified Eagle Medium (DMEM), Roswell Park Memorial Institute medium (RPMI), fetal bovine serum (FBS), and Dulbecco's phosphate-buffered saline (PBS) were purchased from Life Technologies. Highpurity (Milli-Q) water was obtained from an inline Millipore RiOs/Origin water purification system.
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