Il 8 kit

Manufactured by DiaSorin

Sourced in Italy

The IL-8 kit is a laboratory equipment product manufactured by DiaSorin. It is used for the quantitative determination of Interleukin-8 (IL-8) levels in human biological samples.

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Lab products found in correlation

Il 6 kit, by Roche (3 mentions) Il 2r kit, by DiaSorin (3 mentions) Il 10 kit, by DiaSorin (3 mentions) Cobas e602, by Roche (3 mentions) Immulite 1000, by DiaSorin (3 mentions) Il 1β kit, by DiaSorin (2 mentions)

3 protocols using il 8 kit

COVID-19 Cytokine Secretion Profiling

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In order to explore the influence of COVID-19 on the secretion of cytokines, chemiluminescence immunoassay (CLIA) was performed. Cytokines including IL-1β, interleukin 2 receptor (IL-2R), IL-6, IL-8 (also known as CXCL8), IL-10 and TNF were assessed using serum samples drawn from patients shortly after hospitalisation. CLIA was performed using a fully automated analyser (Immulite 1000, DiaSorin Liaison, Italy; or Cobas e602, Roche Diagnostics, Germany) for all the patients according to the manufacturer’s instructions. IL-1β kit (#LKL11), IL-2R kit (#LKIP1), IL-8 kit (#LK8P1), IL-10 kit (#LKXP1) and TNF kit (#LKNF1) were purchased from DiaSorin (Vercelli, Italy). IL-6 kit (#05109442190) was purchased from Roche Diagnostics. The patients were divided into elevated and normal groups according to the instructions.

Liu Q.Q., Cheng A., Wang Y., Li H., Hu L., Zhao X., Wang T, & He F. (2020). Cytokines and their relationship with the severity and prognosis of coronavirus disease 2019 (COVID-19): a retrospective cohort study. BMJ Open, 10(11), e041471.

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Cytokine Profiling in Patient Serum

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According to hospital’s standard procedures, fresh blood samples were centrifuged for 10 min at 2,000g. Serum was collected and tested within 4–6 h. All procedures were performed under level 3 protection. Cytokines including interleukin-2 receptor (sIL-2R), IL-1 β, IL-6, IL-8, IL-10, and TNF-α were assessed in serum samples drawn shortly at each time points by chemiluminescence immunoassay (CLIA) performed on a fully automated analyzer (Immulite 1000, DiaSorin Liaison, Italy or Cobas e602, Roche Diagnostics, Germany) for all patients according to the manufacturer’s instructions. IL-2R kit (#LKIP1), IL-1 β kit (#LKL11), IL-8 kit (#LK8P1), IL-10 kit (#LKXP1), and TNF-α kit (#LKNF1) were purchased from DiaSorin (Vercelli, Italy). IL-6 kit (#05109442 190) was purchased from Roche Diagnostics, Germany.

Han M., Ma K., Wang X., Yan W., Wang H., You J., Wang Q., Chen H., Guo W., Chen T., Ning Q, & Luo X. (2021). Immunological Characteristics in Type 2 Diabetes Mellitus Among COVID-19 Patients. Frontiers in Endocrinology, 12, 596518.

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Cytokine Profiling in Patient Sera

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We detected cytokines, including IL-6, IL-8, IL-10, and IL-2R, in patient serum samples. Assays were conducted using an automated analyzer (Cobas e602, Roche Diagnostics, Germany or Immulite 1000, DiaSorin Liaison, Italy) as described in the manufacturers’ instructions. The IL-6 kit (#05109442190) was obtained from Roche Diagnostics (Mannheim, Germany) and the IL-8 kit (#LK8P1), IL-10 kit (#LKXP1), and IL-2R kit (#LKIP1) were obtained from DiaSorin (Vercelli, Italy).

Wu P., Chen D., Ding W., Wu P., Hou H., Bai Y., Zhou Y., Li K., Xiang S., Liu P., Ju J., Guo E., Liu J., Yang B., Fan J., He L., Sun Z., Feng L., Wang J., Wu T., Wang H., Cheng J., Xing H., Meng Y., Li Y., Zhang Y., Luo H., Xie G., Lan X., Tao Y., Li J., Yuan H., Huang K., Sun W., Qian X., Li Z., Huang M., Ding P., Wang H., Qiu J., Wang F., Wang S., Zhu J., Ding X., Chai C., Liang L., Wang X., Luo L., Sun Y., Yang Y., Zhuang Z., Li T., Tian L., Zhang S., Zhu L., Chang A., Chen L., Wu Y., Ma X., Chen F., Ren Y., Xu X., Liu S., Wang J., Yang H., Wang L., Sun C., Ma D., Jin X, & Chen G. (2021). The trans-omics landscape of COVID-19. Nature Communications, 12, 4543.

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