Advantage hr hr b ccd camera system

For electron microscopic processing, brain sections were post-fixed for 1-hour in a solution of 2% osmium tetroxide (EMS) in PB, followed by embedment in EM BED 812 between 2 sheets of Aclar plastic (Milner et al., 2011 (link)). Trapezoidal sections of the caudal PVN were ultrathin sectioned (60–80 nm) with a diamond knife using an ultramicrotome (Ultratome, NOVA, LKB, Bromma), collected on 400-mesh thin-bar copper grids (EMS). Ultrathin sections of the PVN were analyzed using a transmission electron microscope (Tecnai 12 BioTwin, FEI, Hillsboro, OR) interfaced to a digital camera (Advantage HR/HR-B CCD Camera System, Advanced Microscopy Techniques, Danvers, MA) that was used to collect images from the sampled tissue.

The surface of each flat-embedded forebrain section containing the PVN (Fig. 1) was cut in 60–80 nm sections with a diamond knife using an ultramicrotome (Ultratome, NOVA, LKB, Bromma, Sweden). The ultrathin sections were collected on 400-mesh, thin-bar copper grids (EMS) and counterstained with uranyl acetate and Reynold’s lead citrate (Milner et al., 2011 ). Labeling for TNFR1 in the PVN was analyzed using a transmission electron microscope (Technai 12 BioTwin, FEI, Hillsboro, OR) interfaced to a digital camera (Advantage HR/HR-B CCD Camera System, Advanced Microscopy Techniques, Danvers, MA) that was used to collect digital images from the sampled tissue.