Cobalt affinity resin

Manufactured by Thermo Fisher Scientific

Cobalt affinity resin is a chromatographic material designed for the purification of histidine-tagged recombinant proteins. It utilizes the specific interaction between the histidine tag and the immobilized cobalt ions to capture and separate the target protein from the sample mixture.

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Lab products found in correlation

Isopropyl β d thiogalactopyranoside iptg, by GoldBio (2 mentions) Dna primers, by Integrated DNA Technologies (1 mentions) N decyl β d maltopyranoside, by Anatrace (1 mentions) 1 palmitoyl 2 oleoyl sn glycero 3 phospho 1 rac glycerol, by Avanti Polar Lipids (1 mentions) N nbd dppe, by Avanti Polar Lipids (1 mentions) N rh dppe, by Avanti Polar Lipids (1 mentions) 1 palmitoyl 2 oleoyl sn glycero 3 phosphocholine popc, by Avanti Polar Lipids (1 mentions) 1 palmitoyl 2 oleoyl sn glycero 3 phospho 1 rac glycerol sodium salt popg, by Avanti Polar Lipids (1 mentions)

2 protocols using cobalt affinity resin

Purification and Reconstitution of Membrane Proteins

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Some purchased materials were: DNA primers – Integrated DNA Technologies, Coralville, IA; Escherichia coli BL21(DE3) strain – Novagen, Gibbstown, NJ; Luria-Bertani (LB) medium – Dot Scientific, Burton, MI; isopropyl β-D-thiogalactopyranoside (IPTG) – Goldbio, St. Louis, MO; Cobalt affinity resin – Thermo Scientific, Waltham, MA; n-decyl-β-D-maltopyranoside (DM) – Anatrace, Maumee, OH; 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (sodium salt) (POPG), 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), N-(7-nitro-2,1,3-benzoxadiazol-4-yl) (ammonium salt) dipalmitoylphosphatidylethanolamine (N-NBD-DPPE), N-(lissaminerhodamine B sulfonyl) (ammonium salt) dipalmitoylphosphatidylethanolamine(N-Rh-DPPE) – Avanti Polar Lipids, Alabaster, AL. Most other materials were purchased from Sigma-Aldrich, St. Louis, MO.

Ranaweera A., Ratnayake P.U, & Weliky D.P. (2018). Stabilities of the Soluble Ectodomain and Fusion Peptide Hairpins of the Influenza Virus Hemagglutinin Subunit II Protein Are Positively-Correlated with Membrane Fusion. Biochemistry, 57(37), 5480-5493.

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Recombinant Protein Expression and Purification

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Materials were purchased from the following companies: DNA – GenScript, Piscataway, NJ; Escherichia coli BL21(DE3) strain – Novagen, Gibbstown, NJ; Luria-Bertani (LB) medium – Dot Scientific, Burton, MI; isopropyl β-D-thiogalactopyranoside (IPTG) – Goldbio, St. Louis, MO; Cobalt affinity resin – Thermo Scientific, Waltham, MA; 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (sodium salt) (POPG) – Avanti Polar Lipids, Alabaster, AL. Most other materials were obtained from Sigma-Aldrich, St. Louis, MO.

Liang S., Ratnayake P.U., Keinath C., Jia L., Wolfe R., Ranaweera A, & Weliky D.P. (2018). Efficient Fusion at Neutral pH by Human Immunodeficiency Virus gp41 Trimers containing the Fusion Peptide and Transmembrane Domains. Biochemistry, 57(7), 1219-1235.

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