To analyze the nonspecific immune response after immunization, the serum enzyme activities were determined from 1 to 4 wpi, which included lysozyme (LZM), acid phosphatase (ACP), and alkaline phosphatase (ALP) activities. The detection methods were carried out according to the corresponding enzyme activity test kits (Nanjing Jiancheng, Jiangsu, China).
Enzyme activity test kits
Manufactured by Nanjing Jiancheng
Sourced in China
Enzyme activity test kits are laboratory equipment designed to measure the activity levels of specific enzymes. These kits provide a standardized and quantitative method to determine the catalytic efficiency of enzymes, which is crucial for various applications in biochemistry, biotechnology, and clinical research.
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Lab products found in correlation
2 protocols using enzyme activity test kits
1
Serum Enzyme Activities After Immunization
2
Semen Antioxidant Enzyme Assessment
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Semen samples were centrifuged at 750 rpm for 10 min to remove the supernatants, obtained cell pellets were washed three times with PBS and then incubated with 0.2% Triton X-100 on ice for 20 min. The supernatants were pipetted for subsequent biochemical analyses. Superoxide dismutase (SOD) and Catalase (CAT) activities were examined using the spectrophotometric method by commercially available enzyme activity test kits (Jiancheng Bioengineering Institute, China), Malondialdehyde (MDA) content was determined using the thiobarbituric acid (TBA) method by chemical reaction kits (Jiancheng Bioengineering Institute, China), which performed following manufacturer's instructions as described previously [11] .
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