TEM and cryo-TEM (see Fig. 1b, e , and Fig. S8–S11) was performed on a FEI Sphera microscope operating at 200 keV. TEM grids were prepared by depositing small (3.5 μL) aliquots of sample onto grids (formvar stabilized with carbon (5–10 nm) on 400 copper mesh, Ted Pella Inc.) that had previously been glow discharged using an Emitech K350 glow discharge unit and plasma-cleaned for 90 s in an E.A. Fischione 1020 unit. The sample grid was then stained with 1% uranyl acetate solution, rinsed with water (∼5 μL), and excess liquid was removed. Micrographs were recorded on a 2k × 2k Gatan CCD camera. Samples for cryo-TEM were prepared by depositing 3.5 μL of sample to a freshly glow discharged Quantifoil R2/2 TEM grid. The grids were blotted with filter paper under high humidity to create thin films and rapidly plunged into liquid ethane. The grids were transferred to the microscope under liquid nitrogen and kept at <–175 °C while imaging.
Sphera microscope
Manufactured by Thermo Fisher Scientific
The Sphera microscope is a high-performance optical microscope designed for a wide range of laboratory applications. It features a durable and ergonomic construction, providing a stable platform for precise observations. The Sphera microscope is equipped with advanced optics, delivering clear and detailed images to support various scientific and research activities.
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Lab products found in correlation
2 protocols using sphera microscope
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Transmission Electron Microscopy Protocols
2
Uranyl Acetate Staining for TEM
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A 5 μL
portion of sample was applied to 400 mesh carbon grids (Ted Pella,
Inc., Redding, CA). Grids were glow discharged using an Emitech K350
glow discharge unit and plasma-cleaned for 90 s in an E.A. Fischione
1020 unit. A 5 μL portion of a 1% (w/v) uranyl acetate solution
was applied to each grid for 20 s, and then, the grids were washed
with deionized water and allowed to dry. TEM imaging was performed
on an FEI Sphera microscope operated at 200 keV. Micrographs were
recorded on a 2k × 2k Gatan CCD camera.
portion of sample was applied to 400 mesh carbon grids (Ted Pella,
Inc., Redding, CA). Grids were glow discharged using an Emitech K350
glow discharge unit and plasma-cleaned for 90 s in an E.A. Fischione
1020 unit. A 5 μL portion of a 1% (w/v) uranyl acetate solution
was applied to each grid for 20 s, and then, the grids were washed
with deionized water and allowed to dry. TEM imaging was performed
on an FEI Sphera microscope operated at 200 keV. Micrographs were
recorded on a 2k × 2k Gatan CCD camera.
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