Atenolol (99% pure), betaxolol (96% pure), esmolol (98% pure), timolol (99% pure) and
pindolol (99% pure) were purchased from Abcam (Cambridge, UK), metoprolol (98% pure) was purchased from Alfa Aesar (Ward Hill, MA, USA), and propranolol (99% pure) was purchased from Acros Organic (Morris Plains, NJ, USA). All tested compounds were dissolved in dimethylsulfoxide (DMSO, ≥99%, Ph. Eur.) which was obtained from Sigma-Aldrich Co. (St. Louis, MO, USA).
TrypLE Express, Dulbecco’s modified Eagle high glucose medium (DMEM GlutaMAX),
fetal bovine serum (FBS), penicillin/streptomycin solution (10,000 IU/mL), and
phosphate buffered saline (PBS, pH = 7.4) were purchased from Gibco (Carlsbad, CA, USA). The aqueous 16% paraformaldehyde solution (PFA), the Hoechst 33342 (1 mg/mL) solution, and the
Propidium iodide (1 mg/mL) solution were obtained from Thermo Fisher Scientific (Heysham, UK).
3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, ≥97%) and
crystal violet (≥90%) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Ethanol (96.6%) was obtained from Stumbras, LLC (Kaunas, Lithuania).
All cell culture plastic ware was purchased from Thermo Fisher Scientific, Corning (Phoenix, AZ, USA) and Techno Plastic Products (Trasadingen, Switzerland).
Sidorova M, & Petrikaitė V. (2022). The Effect of Beta Adrenoreceptor Blockers on Viability and Cell Colony Formation of Non-Small Cell Lung Cancer Cell Lines A549 and H1299. Molecules, 27(6), 1938.
