Gly l phe d thr gly l phe l leu l ser o β d glucose conh2 g7

Poly(D,L-lactide-co-glycolide) (PLGA,RG503H, MW near 11,000) was used as received from the manufacturer (Boehringer-Ingelheim, Ingelheim am Rhein, Germany). Polyvinyl alcohol (PVA, MW 15,000) was purchased from Sigma-Aldrich (Milan, Italy). Gly-L-Phe-D-Thr-Gly-L-Phe-L-Leu-L-Ser(O-β-D-Glucose)-CONH2 (g7) linked to biotin was synthesized as previously reported and purchased from Mimotopes (Clayton, Victoria, Australia) [14 (link)]. PLGA conjugated with Rhodamine B piperazine amide (Sigma-Aldrich) (R-PLGA) was prepared as previously described [28 (link)]. Albumin-fluorescein isothiocyanate conjugate (FITC-Albumin) was purchased from Sigma-Aldrich (A9771). Maleimide activated Neutravidin (NA-Maleido) was obtained by Thermo Scientific. A MilliQ water system (Millipore, Bedford, MA, USA), supplied with distilled water, provided high-purity water (18 MΩ). All other chemicals were of analytical grade.

Poly(D,L-lactide-co-glycolide) (PLGA, RG503H, MW near 11,000) was used as received from the manufacturer (Boehringer-Ingelheim, Ingelheim am Rhein, Germany). Polyvinyl alcohol (PVA, MW 15,000) was purchased from Sigma-Aldrich (Milan, Italy). Gly-L-Phe-D-Thr-Gly-L-Phe-L-Leu-L-Ser(O-β-D-Glucose)-CONH2 (g7) linked to PLGA was synthesized as previously reported [26 (link)] and purchased from Mimotopes (Clayton, Victoria, Australia). Trehalose dihydrate (MW 378.33) was purchased from Sigma-Aldrich and used as cryoprotectant. A MilliQ water system (Millipore, Bedford, MA, USA) supplied with distilled water provided high-purity water (18 MΩ). All the other chemicals were of analytical grade.