For physiological index measurements, the content of malondialdehyde (MDA), proline (Pro), Chlorophyll, and the activities of catalase (CAT) were determined with Malondialdehyde (MDA) assay kit, proline assay kit, Chlorophyll assay kit, Catalase (CAT) assay kit, and Peroxidase assay kit (Nanjing Jiancheng, Nanjing, China), respectively. All samples were taken from the third leaf of tobacco.
Chlorophyll assay kit
Manufactured by Nanjing Jiancheng
Sourced in China
The Chlorophyll assay kit is a laboratory equipment designed to quantify the amount of chlorophyll present in plant samples. It provides a straightforward method for the extraction and measurement of chlorophyll content, which is a key indicator of plant health and photosynthetic activity.
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Lab products found in correlation
Malondialdehyde mda assay kit, by Nanjing Jiancheng (1 mentions)
Catalase cat assay kit, by Nanjing Jiancheng (1 mentions)
Peroxidase assay kit, by Nanjing Jiancheng (1 mentions)
Proline assay kit, by Nanjing Jiancheng (1 mentions)
Epoch microplate spectrophotometer, by Agilent Technologies (1 mentions)
Mda assay kit, by Nanjing Jiancheng (1 mentions)
Total antioxidant capacity assay kit, by Nanjing Jiancheng (1 mentions)
3 protocols using chlorophyll assay kit
1
Tobacco Leaf Physiological Indices
2
Chlorophyll and MDA Analysis in Tea
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The chlorophyll assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) was used to determine the content of total chlorophylls in different tea samples. Briefly, the tea leaves (0.1 g) were ground in liquid nitrogen with 1 mL distilled water and 50 mg absorbent in dark, 10 mL of extraction solution [ethanol:acetone = 1:2 (v/v)] was added and kept until the sediment turned white in dark. The extract was filtered and the filtrate was analyzed using Epoch microplate spectrophotometer (Bio Tek, Winooski, VT, USA) at the wavelengths of 645 and 663 nm.
The plant malondialdehyde (MDA) assay kit (colorimetric method) was purchased from Nanjing Jiancheng Bioengineering Institute, Nanjing, China. The tea leaves (0.1 g) were ground in liquid nitrogen, then the powder was transferred to a 1.5 mL centrifuge tube, followed by the addition of 0.9 mL extraction buffer. After homogenization, the supernatant was centrifuged at 8000 rpm and 4 °C for 10 min. Further, the supernatant reacted with thiobarbituric acid (TBA) according to the protocol, and the absorbance of mixture was analyzed by Epoch microplate spectrophotometer (Bio Tek, Winooski, VT, USA) at the wavelength of 532 nm.
The plant malondialdehyde (MDA) assay kit (colorimetric method) was purchased from Nanjing Jiancheng Bioengineering Institute, Nanjing, China. The tea leaves (0.1 g) were ground in liquid nitrogen, then the powder was transferred to a 1.5 mL centrifuge tube, followed by the addition of 0.9 mL extraction buffer. After homogenization, the supernatant was centrifuged at 8000 rpm and 4 °C for 10 min. Further, the supernatant reacted with thiobarbituric acid (TBA) according to the protocol, and the absorbance of mixture was analyzed by Epoch microplate spectrophotometer (Bio Tek, Winooski, VT, USA) at the wavelength of 532 nm.
3
Measuring Leaf Biochemical Profiles
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The total soluble sugar contents of CK, UVB, and UVD were measured
with a plant soluble sugar content test kit (Solarbio, Beijing, China)
according to the instructions. The absorbance of each sample was measured
at 620 nm, and the content of soluble sugar was calculated on the
basis of a standard curve. The T-AOC and chlorophyll content of each
group were determined colorimetrically by a total antioxidant capacity
assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China)
and chlorophyll assay kit (Nanjing Jiancheng Bioengineering Institute).
Chlorophyll content determination was performed with 0.1 g leaf samples.
The absorbance values at OD645 and OD663 were
measured. The calculation formula are as follows: (1) chlorophyll
a = (12.7 × OD663 – 2.69 × OD645) × volume × dilution rate/(sample weight × 1000).
(2) Chlorophyll b = (22.9 × A645 – 4.68 × A663) ×
volume×dilution rate/(sample weight × 1000). (3) Total chlorophyll
content = chlorophyll a content + chlorophyll b content. T-AOC content
measurement was performed after incubating the sample and mix buffer
at 37 °C for 30 min. At least three independent biological replicates
were performed per treatment.
with a plant soluble sugar content test kit (Solarbio, Beijing, China)
according to the instructions. The absorbance of each sample was measured
at 620 nm, and the content of soluble sugar was calculated on the
basis of a standard curve. The T-AOC and chlorophyll content of each
group were determined colorimetrically by a total antioxidant capacity
assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China)
and chlorophyll assay kit (Nanjing Jiancheng Bioengineering Institute).
Chlorophyll content determination was performed with 0.1 g leaf samples.
The absorbance values at OD645 and OD663 were
measured. The calculation formula are as follows: (1) chlorophyll
a = (12.7 × OD663 – 2.69 × OD645) × volume × dilution rate/(sample weight × 1000).
(2) Chlorophyll b = (22.9 × A645 – 4.68 × A663) ×
volume×dilution rate/(sample weight × 1000). (3) Total chlorophyll
content = chlorophyll a content + chlorophyll b content. T-AOC content
measurement was performed after incubating the sample and mix buffer
at 37 °C for 30 min. At least three independent biological replicates
were performed per treatment.
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