Gem hydrochloride

Manufactured by Merck Group

Sourced in United States

GEM hydrochloride is a laboratory reagent used for various scientific applications. It is a crystalline solid compound that dissolves in water and other polar solvents. The core function of GEM hydrochloride is to serve as a chemical building block and intermediate in the synthesis of other compounds.

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Lab products found in correlation

Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)

Close spelling variants of this product

Hydrochlorides

3 protocols using gem hydrochloride

Characterization of PLGA Polymer

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GEM hydrochloride was obtained from Sigma-Aldrich Co. (St Louis, MO, USA). OP with 98% purity was obtained from Hangzhou DayangChem Co., Ltd (Hangzhou, China). PLGA (50/50) was obtained from Purac Biomaterials (Gorinchem, the Netherlands). The molecular weight was determined by gel permeation chromatography (Viscotek GPCmas VE 2001; Malvern Instruments, Malvern, UK) and calibrated with poly(ethylene oxide) standards. PLGA was determined to have a molecular weight (M_W) of 16,400 (M_W/M_n =1.58).

Allison Logan S., Brissenden A.J., Szewczuk M.R, & Neufeld R.J. (2017). Combinatorial and sequential delivery of gemcitabine and oseltamivir phosphate from implantable poly(d,l-lactic-co-glycolic acid) cylinders disables human pancreatic cancer cell survival. Drug Design, Development and Therapy, 11, 2239-2250.

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Combinatorial effects of PRO and GEM

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Cell lines were treated for 48 h with different concentrations (0.003–300 µM) of PRO hydrochloride (Sigma-Aldrich) and/or (0.05–150 μM) of GEM hydrochloride (Sigma-Aldrich), or with different combinations of PRO and GEM as indicated in the experiments. PRO and GEM dilutions were obtained from a 10⁻² M stock solution prepared in DMSO (Sigma-Aldrich). For the combined treatments, PRO and GEM were simultaneously added to complete medium. Control cells were treated with corresponding volumes of DMSO. The treatments were renewed every 24 h.

Garcia-Gil M., Turri B., Gabriele M., Pucci L., Agnarelli A., Lai M., Freer G., Pistello M., Vignali R., Batistoni R, & Marracci S. (2021). Protopine/Gemcitabine Combination Induces Cytotoxic or Cytoprotective Effects in Cell Type-Specific and Dose-Dependent Manner on Human Cancer and Normal Cells. Pharmaceuticals, 14(2), 90.

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Synthesis and Characterization of GEM Hydrochloride

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All chemicals were purchased from Sigma-Aldrich unless otherwise specified and were used as received. GEM hydrochloride (United States Pharmacopeia reference standard) was purchased from Sigma (catalog no. 1288463). aPDL1 used in vivo was purchased from BioLegend Inc. (catalog no. 124329, clone 10F.9G2).

Wang C., Wang J., Zhang X., Yu S., Wen D., Hu Q., Ye Y., Bomba H., Hu X., Liu Z., Dotti G, & Gu Z. (2018). In situ formed reactive oxygen species-responsive scaffold with gemcitabine and checkpoint inhibitor for combination therapy. Science translational medicine, 10(429).

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