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Lc3b ab51520

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LC3B-ab51520 is a polyclonal antibody produced in rabbit that recognizes the light chain 3B (LC3B) protein. LC3B is a key autophagy marker that is essential for the formation of autophagosomes during the autophagic process.

Automatically generated - may contain errors

Lab products found in correlation

Pink1 ab23707, by Santa Cruz Biotechnology (3 mentions) P62 sqstm1 ab56416, by Santa Cruz Biotechnology (3 mentions) Ab 54481, by Santa Cruz Biotechnology (2 mentions) Ppargc1a sc 13067, by Santa Cruz Biotechnology (2 mentions) Dio2 sc 98716, by Santa Cruz Biotechnology (2 mentions) Bcl xl ab32124, by Santa Cruz Biotechnology (2 mentions) Bax ab32503, by Santa Cruz Biotechnology (2 mentions) β actin sc 47778, by Santa Cruz Biotechnology (1 mentions)

3 protocols using lc3b ab51520

1

Protein Expression Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteins from tissues or cultured cells were extracted, transferred onto
PVD-membranes, hybridized overnight with appropriate primary antibodies
(PPARGC1A - sc-13067 and ab-54481, DIO2-sc-98716, BCL-xL- ab32124,
PINK1-ab23707, LC3B-ab51520, p62/SQSTM1-ab56416, BAX-ab32503 and ab-53154,
β-actin-sc-47778) obtained from Santa Cruz Biotechnology, CA (sc), or
Abcam, Cambridge, UK (ab) and visualized using the Gel Doc XR+ System
(Biorad, Lab. Inc. Life Science, USA), according to manufacturer’s
protocol.
Yu G., Tzouvelekis A., Wang R., Herazo-Maya J.D., Ibarra G.H., Srivastava A., de Castro J.P., DeIuliis G., Ahangari F., Woolard T., Aurelien N., e Drigo R.A., Gan Y., Graham M., Liu X., Homer R.J., Scanlan T.S., Mannam P., Lee P.J., Herzog E.L., Bianco A.C, & Kaminski N. (2017). Thyroid hormone inhibits lung fibrosis in mice by improving epithelial mitochondrial function. Nature medicine, 24(1), 39-49.
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2

Protein Extraction and Immunoblotting

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total protein extracts were isolated from the cells upon mentioned treatments using common procedures, transferred into PVD-membranes, and hybridized overnight using the following primary antibodies (PINK1-ab23707, LC3B-ab51520, β-actin-sc-47778, and p62/SQSTM1-ab56416) obtained from Santa Cruz Biotechnology, CA (sc), or Abcam, Cambridge, UK (ab).
Karampitsakos T., Galaris A., Barbayianni I., DeIuliis G., Ahangari F., Sampsonas F., Sotiropoulou V., Aidinis V., Bennett A.M., Herazo-Maya J.D., Xylourgidis N., Bakakos P., Bouros D., Kaminski N, & Tzouvelekis A. (2023). SH2 Domain-Containing Phosphatase-SHP2 Attenuates Fibrotic Responses through Negative Regulation of Mitochondrial Metabolism in Lung Fibroblasts. Diagnostics, 13(6), 1166.
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3

Protein Expression Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteins from tissues or cultured cells were extracted, transferred onto
PVD-membranes, hybridized overnight with appropriate primary antibodies
(PPARGC1A - sc-13067 and ab-54481, DIO2-sc-98716, BCL-xL- ab32124,
PINK1-ab23707, LC3B-ab51520, p62/SQSTM1-ab56416, BAX-ab32503 and ab-53154,
β-actin-sc-47778) obtained from Santa Cruz Biotechnology, CA (sc), or
Abcam, Cambridge, UK (ab) and visualized using the Gel Doc XR+ System
(Biorad, Lab. Inc. Life Science, USA), according to manufacturer’s
protocol.
Yu G., Tzouvelekis A., Wang R., Herazo-Maya J.D., Ibarra G.H., Srivastava A., de Castro J.P., DeIuliis G., Ahangari F., Woolard T., Aurelien N., e Drigo R.A., Gan Y., Graham M., Liu X., Homer R.J., Scanlan T.S., Mannam P., Lee P.J., Herzog E.L., Bianco A.C, & Kaminski N. (2017). Thyroid hormone inhibits lung fibrosis in mice by improving epithelial mitochondrial function. Nature medicine, 24(1), 39-49.
+ Open protocol
+ Expand

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