Anti rabbit igg h l secondary antibody

Manufactured by Jackson ImmunoResearch

Sourced in United States

Anti-Rabbit IgG (H+L) Secondary Antibody is a reagent used in immunochemical techniques to detect and visualize the presence of rabbit primary antibodies. It is specifically designed to bind to the heavy and light chains of rabbit immunoglobulin G (IgG), allowing for the identification and localization of target antigens in biological samples.

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Lab products found in correlation

Ripa buffer, by Thermo Fisher Scientific (4 mentions) Easypack protease inhibitors, by Roche (2 mentions) Anti mouse igg h l secondary antibody, by Jackson ImmunoResearch (2 mentions) Trans blot turbo transfer system, by Bio-Rad (2 mentions) Fstl1, by R&D Systems (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) Ab16039, by Abcam (1 mentions) Ab5694, by Abcam (1 mentions)

2 protocols using anti rabbit igg h l secondary antibody

Fstl1 Protein Quantification via Western Blot

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Tissue samples (50 mg) were solubilized in Ripa Buffer (89900, Thermo Fisher, Waltham, MA, USA) with addition of EASYpack protease inhibitors (5892970001, Roche). Protein concentration in supernatants after 14,000 rpm centrifugation was determined using the BCA method. 25 mkg of protein were separated on 8% SDS-PAGE with subsequent blotting on nitrocellulose membrane (BIO-RAD) using Trans-Blot^® Turbo™ Transfer System (Bio-Rad) with subsequent immersion in 5% skimmed milk in TBST buffer.
Primary ab: 1:1000-FSTL1, (BAF1738, R&D System); GAPDH (32233, Santa-Cruz). Secondary ab: 1:10000-anti-Mouse IgG (H+L) Secondary Antibody (115-005-003, Jackson ImmunoResearch), anti-Rabbit IgG (H+L) Secondary Antibody (111-035-144, Jackson ImmunoResearch). Signal was revealed by the ECL method following the HRP reaction.

Andreichenko I.N., Tsitrina A.A., Fokin A.V., Gabdulkhakova A.I., Maltsev D.I., Perelman G.S., Bulgakova E.V., Kulikov A.M., Mikaelyan A.S, & Kotelevtsev Y.V. (2019). 4-methylumbelliferone Prevents Liver Fibrosis by Affecting Hyaluronan Deposition, FSTL1 Expression and Cell Localization. International Journal of Molecular Sciences, 20(24), 6301.

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Quantitative Western Blot Analysis of αSMA

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Tissue samples (50 mg) were solubilized in Ripa Buffer (89900, Thermo Fisher) with the addition of EASYpack protease inhibitors (5892970001, Roche, Switzerland). Protein concentration in supernatants after 14,000 rpm centrifugation was determined using the BCA method. 25 μg of protein were separated on 8% SDS-PAGE with subsequent blotting on a nitrocellulose membrane (BIO-RAD, USA) using Trans-Blot® Turbo™ Transfer System (Bio-Rad, USA) with subsequent immersion in 5% skimmed milk in TBST buffer. Primary ab: anti-αSMA (ab5694, Abcam, UK); anti-beta Actin (ab16039, Abcam, UK). Secondary ab: 1:10,000-anti-Mouse IgG (H + L) secondary antibody (115-005-003, Jackson ImmunoResearch, USA), anti-Rabbit IgG (H + L) secondary antibody (111-035-144, Jackson ImmunoResearch, USA). Signal was revealed by the ECL method following the HRP reaction.

Tsitrina A.A., Krasylov I.V., Maltsev D.I., Andreichenko I.N., Moskvina V.S., Ivankov D.N., Bulgakova E.V., Nesterchuk M., Shashkovskaya V., Dashenkova N.O., Khilya V.P., Mikaelyan A, & Kotelevtsev Y. (2021). Inhibition of hyaluronan secretion by novel coumarin compounds and chitin synthesis inhibitors. Glycobiology, 31(8), 959-974.

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