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Incucyte scratch wound cell migration and invasion system

Manufactured by Sartorius
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The IncuCyte® Scratch Wound Cell Migration and Invasion System is a lab equipment that enables automated, real-time monitoring and analysis of cell migration and invasion in a controlled environment. It provides quantitative data on cell behavior over time.

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Lab products found in correlation

Incucyte zoom analysis software, by Sartorius (1 mentions) Mitomycin c, by Merck Group (1 mentions) Incucyte zoom kinetic imaging system, by Sartorius (1 mentions) Wound making tool, by Sartorius (1 mentions)

2 protocols using incucyte scratch wound cell migration and invasion system

1

Measuring Cell Migration using Scratch Assay

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Approximately 100,000 cells per well were seeded in 96-well ImageLock plates, yielding 90–100% confluency 24 hours after plating. To inhibit proliferation, cells were treated with 2 µg/ml mitomycin C (Sigma) for 1 hour and wounds generated using the Incucyte wound maker [19] . After rinsing with phosphate-buffered saline 3 times, cells were further cultured in the IncuCyte® Scratch Wound Cell Migration and Invasion System (Essen BioScience) and images were taken every hour during the 24 hour incubation. The relative percentage of wound area occupied by migrated cells was calculated relative to the original wound area using IncuCyte ZOOM analysis software (Essen BioScience).
Chen D., Cox J., Annam J., Weingart M., Essien G., Rathi K.S., Rokita J.L., Khurana P., Cuya S.M., Bosse K.R., Pilgrim A., Li D., Shields C., Laur O., Maris J.M, & Schnepp R.W. (2020). LIN28B promotes neuroblastoma metastasis and regulates PDZ binding kinase. Neoplasia (New York, N.Y.), 22(6), 231-241.
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2

Wound Healing Assay with PRI-724

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Thirty thousand of NTERA-2 CisR cells (untreated or pretreated with PRI-724 inhibitor for 72 h) per well were plated in pentaplicates in ECM-coated ImageLock 96-well plates (Essen BioScience, Royston, UK) and let to adhere overnight. Confluent monolayers were wounded with wound making tool (Essen BioScience), washed and supplemented with serum-free culture medium with or without PRI-724 inhibitor (1.25 μM). Cell migration was evaluated by IncuCyte® Scratch Wound Cell Migration and Invasion System and documented by the IncuCyte ZOOM™ kinetic imaging system (Essen BioScience). Results were based on the relative wound density measurements and expressed as means of three independent experiments run in pentaplicates ± SD.
Schmidtova S., Kalavska K., Liskova V., Plava J., Miklikova S., Kucerova L., Matuskova M., Rojikova L., Cierna Z., Rogozea A., Konig H., Albany C., Mego M, & Chovanec M. (2021). Targeting of Deregulated Wnt/β-Catenin Signaling by PRI-724 and LGK974 Inhibitors in Germ Cell Tumor Cell Lines. International Journal of Molecular Sciences, 22(8), 4263.
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