Bca test kit

HCC cell line (Hep3B) was purchased from Shanghai Institutes for Biological Sciences (Shanghai, China); Modified Eagle’s Medium (MEM) and penicillin/streptomycin (PS) were purchasd from Gibco Biotechnology Co., Ltd. (Beijing, China); Fetal bovine serum (FBS) was purchased from Hyclone Bioscience Co., Ltd. (Beijing, China); Extract total RNA, first-stand cDNA reverse transcription and polymerase chain reaction kits were purchased from TianGen Biotechnology Co., Ltd. (Beijing, China); Cellular free fatty acid test kit was purchased from Solarbio Biotechnology Co., Ltd. (Beijing, China); Rabbit anti-SREBP1 (bs-1402R) was purchased from Bioss Bioscience Co., Ltd. (Beijing, China); Rabbit anti-FASN (ab22759), rabbit anti-ACC (ab45174) and rabbit anti-ACLY (ab40793) and HRP-conjugate goat anti-rabbit IgG (ab205718) were purchased from Abcam bioscience Co., Ltd. (Shanghai, China); Rabbit anti-β-actin (20536–1-AP) was obtained from Proteintech, Inc.,(Wuhan, China); BCA test kit was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Oil Red O Staining Kit was purchased from Solarbio Biotechnology Co., Ltd. (Beijing, China).

Dextran sulfate sodium (DSS, molecular weight 36–50 kDa) was bought from MP Biomedical Corporation (Santa Ana, California, USA). Carboxyl methyl cellulose-Na (CMC-Na) was gained from Shanghai Changguang Enterprise Development Co., Ltd. (Shanghai, China). AIN93G purified rodent diet was acquired from Jiangsu Xietong Pharmaceutical Bioengineering Co., Ltd. (Jiangsu, China). Hemoccult Kits, MPO test kits, BCA test kits were received from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Antibodies for NF-κB p65, IκB-α and phosphor-IκB-α were obtained from Thermo Scientific Co., Ltd. (Waltham, MA, USA). Trizol reagents were acquired from Invitrogen (Carlsbad, CA, USA). Revert Aid reverse transcription kits, PCR SuperMix, RNase-Free Water were purchased from Thermo Scientific Co., Ltd. (Waltham, MA, USA).