D psicose

All enzymes involved in the molecular cloning experiments were purchased from New England Biolabs (NEB). All synthetic oligonucleotides were synthesized by Integrated DNA Technologies. Sanger Sequencing was provided by Genewiz. D-Psicose and D-mannose were purchased from Sigma-Aldrich. D-Glucose was purchased from Fisher Scientific.

Fresh longan fruits cultivar Edor with size code 2 (more than 2.2–2.5 cm in diameter) was purchased from a local market in Lamphun province in 2019. A standard solution of amino acids mixture (type H), which contained 17 common amino acids (e.g., Asp-aspartic acid, Thr-threonine, Ser-serine, Glu-glutamic acid, Pro-proline, Gly-glycine, Ala-alanine, Cys-cysteine, Val-valine, Met-methionine, Ile-isoleucine, Leu-leucine, Tyr-tyrosine, Phe-phenylalanine, His-histidine, Lys-lysine and Arg-arginine) was purchased from Wako Pure Chemical Corporation, Osaka, Japan. Eight types of sugar standards used for analysis were D-psicose, D-glucose, D-fructose, D-xylose, D-rhamnose, D-allose, D-mannose, and sucrose (Sigma-Aldrich Singapore, Singapore). HPLC grade methanol and acetonitrile were purchased from RCI, Labscan, Bangkok, Thailand. All solutions and dilutions were performed using deionized water, which was generated using a Milli-Q water system (Zeneer up 900, Seoul, Korea).