Pcdna pparγ

PFOS (potassium salt; >98% pure) was purchased from MAYA-R (Jiaxing, China). Dimethyl sulfoxide (DMSO) and the cell counting kit-8 (CCK-8) were purchased from Vicmed (Busan, Korea). MEM medium was purchased from Corning (Shanghai, China). DMEM/F12 medium and RPMI 1640 medium were purchased from KeyGEN BioTECH (Nanjing, China). Fetal bovine serum was purchased from ExCell Bio. HiScript^® II Q RT SuperMix for RT-PCR and AceQ^® RT-PCR were obtained from Vazyme (Nanjing, China). Rosiglitazone and GW9662 were purchased from MedChemExpress (Shanghai, China). pcDNA-PPARγ and siRNAs were obtained or synthesized by GenePharma (Shanghai, China). Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA) was purchased from Invitrogen and used for transient transfection.

The si-PPARγ (50 nM), si-Control (50 nM) (GenePharma, Shanghai, China), pcDNA-PPARγ (2 μg), and pcDNA 3.1 (2 μg) (GenePharma, Shanghai, China) were transfected using the Lipofectamine 2000 reagent in six-well culture plates. To achieve PPARγ knockdown and overexpression in HTR-8/SVneo and JEG-3, cells were cultured in six-well plates for 24 h and after 4 h of transfection, DMEM/F12 and MEM supplemented with 10% FBS was added for 24 h.