The medium used for culturing the breast cancer organoid cultures contained the following ingredients: 50% conditioned medium from L-WRN cells (ATCC #CRL-3276) (Miyoshi and Stappenbeck, 2013 (link)) (containing Wnt3a, R-spondin 3, and Noggin), Heregulin 5 nmol/L (Peprotech, NJ, USA), fibroblast growth factor 7 (FGF7) 5 ng/ml (Peprotech), fibroblast growth factor 10 (FGF10) 20 ng/ml (Peprotech), epidermal growth factor (EGF) 5 ng/ml (Peprotech), A83-01 500 nmol/L (Tocris, Wiesbaden, Germany), Y27632 5 µmol/L (Hölzel), SB202190 (Sigma-Aldrich), Gibco B27 Supplement 2% (Thermo Fisher Scientific), N-acetyl-cysteine 1.25 mmol/L (Sigma-Aldrich), nicotinamide 5 mmol/L (Sigma-Aldrich), Primocin 50 µg/ml (InvivoGen, Toulouse, France), Gibco advDMEM/F12 50% (Thermo Fisher Scientific).
Fibroblast growth factor 10 fgf10
Manufactured by Thermo Fisher Scientific
Sourced in United States
Fibroblast growth factor 10 (FGF10) is a protein that plays a role in the regulation of cell growth and differentiation. It is a member of the fibroblast growth factor family, which are involved in various biological processes such as embryonic development, cell growth, morphogenesis, tissue repair, and tumor growth. FGF10 is a key regulator of epithelial-mesenchymal interactions and is essential for the development of several organs, including the lungs, pancreas, and salivary glands.
Automatically generated - may contain errors
Lab products found in correlation
N acetylcysteine, by Merck Group (3 mentions)
Primocin, by InvivoGen (2 mentions)
Epidermal growth factor (egf), by Thermo Fisher Scientific (2 mentions)
A83 01, by Bio-Techne (2 mentions)
Nicotinamide, by Merck Group (2 mentions)
R spondin 1, by Thermo Fisher Scientific (2 mentions)
Y 27632, by Merck Group (2 mentions)
Heregulin, by Thermo Fisher Scientific (1 mentions)
Sb202190, by Merck Group (1 mentions)
Forskolin, by Bio-Techne (1 mentions)
Gastrin, by Merck Group (1 mentions)
Ultraglutamine, by Thermo Fisher Scientific (1 mentions)
Advanced dmem f12, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Hepes, by Thermo Fisher Scientific (1 mentions)
Vascular endothelial growth factor (vegf), by Thermo Fisher Scientific (1 mentions)
Forskolin, by MedChemExpress (1 mentions)
Nicotinamide, by Selleck Chemicals (1 mentions)
Hydrocortisone, by MedChemExpress (1 mentions)
A83 01, by MedChemExpress (1 mentions)
4 protocols using fibroblast growth factor 10 fgf10
1
Breast Cancer Organoid Culture Protocol
2
Liver Organoid Culture Protocol
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The isolation and culture of organoids were conducted using liver tissues obtained during liver transplantation at the Erasmus Medical Center Rotterdam. The utilization of liver tissues for research was approved by the Medical Ethical Council of the Erasmus MC, with informed consent obtained from donors (MEC-2014-060). Organoids were cultured in organoid expansion medium (EM) composed of advanced DMEM/F12 (Invitrogen, USA) supplemented with 1% penicillin/streptomycin (Life Technologies, USA), 1 mol/L Hepes (Life Technologies, USA), 200 mmol/L ultraglutamine (Life Technologies, USA), 1% (v/v) of N2 (Gibco), 2% (v/v) of B27 (Gibco, USA), 1 mmol/L N-acetylcysteine (Sigma-Aldrich, USA), 10 mmol/L nicotinamide (Sigma-Aldrich, USA), 5 μmol/L A83.01 (Tocris, UK), 10 μmol/L forskolin (Tocris, UK), 10 nmol/L gastrin (Sigma-Aldrich, USA), epidermal growth factor (EGF) (50 ng/mL; PeproTech, USA), 10% (v/v) of R-spondin-1 (conditioned medium), fibroblast growth factor 10 (FGF10) (100 ng/mL; PeproTech, USA), hepatocyte growth factor (HGF) (25 ng/mL; PeproTech, USA), and 10 μmol/L Y27632 (Sigma-Aldrich, USA).
3
Co-culture of HepLPCs and HUVECs
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The proliferating HepLPCs and HUVECs were dissociated with TrypLE Express, neutralized with pre-warmed medium, and washed in PBS. 2 × 105 proliferative cells (HepLPCs/HUVEC, 1:1) per well were seeded in ultra-low attachment 6-well-plates. The culture media was based on Advance DMEM/F12 supplemented with GlutaMAX-I, HEPES, Primocin, 1 × B27, 1.56 mM N-Acetylcysteine, 0.5 μm A83-01, 10 μm Y27632, 3 μm CHIR-99021, 50 ng/ml EGF and 25 ng/ml HGF. Due to the HUVEC growth conditions, we supplemented the media with 25 ng/ml vascular endothelial growth factor (VEGF, PeproTech), 10 ng/ml Fibroblast Growth Factor-10 (FGF10, PeproTech), and 1% FBS. Primary spheroids had formed by the next day. The medium was replaced every two or 3 days.
4
Establishing 3D Organoid Culture of Triple-Negative Breast Cancer
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TNBC tissues were cut into small pieces and mechanically homogenised. The homogenised tissue was digested with trypsin on ice. After centrifugation, single‐cell suspensions were added dropwise with cold 3D Matrigel matrix (Corning, 354348) to six‐well culture plates, which were kept inverted in a cell incubator for 30 min before organoid‐specific medium was added. Organoids were cultured with media containing 1% GlutaMax (Thermo Fish, 35050061), 100 μg/mL Primocin (InvivoGen, 26‐69‐PM), .5 μg/mL hydrocortisone (MedChemExpress, HY‐N0583), 10 μM Y27632 (Sigma, Y0503), .2 nM Wnt3a (StemRD, W3a‐H‐025), 250 ng/mL R‐spondin1 (PeproTech, 120‐38), 100 ng/mL Noggin (PeproTech, 120‐10D), 1× B27 + Vitamin A (Invitrogen, 17504‐044), 10 mM HEPES (Thermo Fish, 15630080), 20 ng/mL Fibroblast Growth Factor‐10 (FGF‐10) (Peprotech, 100‐26), 5 ng/mL Epidermal Growth Factor (EGF) (Peprotech, GMP100‐15), 10 mM nicotinamide (Selleckchem, S1899), 100 nM β‐estradiol (Sigma, E2758), 10 μM Forskolin (MedChemExpress, HY‐15371), 5 nM Heregulin B1 (ProSpec, CYT‐733), .5 μM A83‐01 (MedChemExpress, HY‐10432), .5 μM SB202190 (Selleckchem, S10077), 1.25 mM N‐acetylcysteine (Sigma, A0737) and Dulbecco's Modified Eagle Medium (DMEM).
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