Improvision

Manufactured by PerkinElmer

Sourced in United Kingdom

Improvision is a versatile software platform that enables advanced imaging and image analysis for scientific research. It provides a comprehensive suite of tools for capturing, processing, and analyzing high-quality images from a variety of microscopy techniques.

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Close spelling variants of this product

Improvision UltraVIEW VoX system (9 citations) Improvision Volocity software (4 citations) Improvision Openlab (2 citations)

2 protocols using improvision

3D Co-culture Assay with Fibroblasts and 621-101 Cells

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Fibroblasts and 621–101 cells were labelled with CellTracker dyes as above. A total of 10⁵ fibroblasts, 621–101 cells or 5:1 mixture of fibroblasts and 621–101 cells was embedded in 400μl of basement membrane extract (BME, Trevigen, AMS Biotechnology, Abingdon UK) in DME-F12 at a final concentration of 3.75mg/ml in 24 well plates. AMD3100 was included at 200 μg/ml where indicated. After an initial 24 hours incubation, gels were overlaid with 300μl DME-F12 with or without 200μg/ml AMD3100. This medium was replaced at 24h intervals.
Cells within the gels were imaged at 24 hour intervals, for up to 7 days, using a spinning disk confocal microscope (Improvision, Perkin Elmer UK).

Clements D., Dongre A., Krymskaya V.P, & Johnson S.R. (2015). Wild Type Mesenchymal Cells Contribute to the Lung Pathology of Lymphangioleiomyomatosis. PLoS ONE, 10(5), e0126025.

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Tissue Histology for Tumor Biodistribution

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The tumors visualized with the In-Vivo FX PRO system for the biodistribution analyses were immediately fixed in cold 4% paraformaldehyde and stored at 4°C for 48 hours. After incubation, specimens were transferred in 30% sucrose in PBS for 72 hours and then embedded in Optimal Cutting Temperature compound (OCT). Cryosection slides of 10μm were cut by the Research Histology Department at Yale. Tissue was stained with Hoechst 33328 for 10min (1:5000 RT). Images were captured using an Axio Observer.Z1 inverted fluorescence microscope and analyzed with velocity software (Improvision, PerkinElmer).

Cocco E., Deng Y., Shapiro E.M., Bortolomai I., Lopez S., Lin K., Bellone S., Cui J., Menderes G., Black J.D., Schwab C.L., Bonazzoli E., Yang F., Predolini F., Zammataro L., Altwerger G., de Haydu C., Clark M., Alvarenga J., Ratner E., Azodi M., Silasi D.A., Schwartz P.E., Litkouhi B., Saltzman W.M, & Santin A.D. (2016). Dual-targeting nanoparticles for in vivo delivery of suicide genes to chemotherapy-resistant ovarian cancer cells. Molecular cancer therapeutics, 16(2), 323-333.

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