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Cls 2 kit

Manufactured by Roche
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Sourced in Germany

The CLS II KIT is a laboratory equipment product designed for cell counting and cell viability analysis. It provides accurate and reliable cell count measurements using a Coulter-based technology. The CLS II KIT is a core function tool for routine cell analysis in various research and clinical applications.

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Lab products found in correlation

Synergy ht plate reader, by Agilent Technologies (2 mentions)

Spelling variants of this product

ATP Bioluminescence Assay Kit CLS II (166 citations) CLS II (37 citations) ATP Bioluminescence Kit CLS II (11 citations) Bioluminescence Assay Kit CLS II (6 citations) Luciferin/luciferase ATP bioluminescence assay kit CLS II (6 citations)

4 protocols using cls 2 kit

1

Quantifying Memory T-cell ATP Levels

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According to the manufacturer’s instructions, the ATP content of isolated memory Th cells was assessed using the CLS II KIT (Roche, Mannheim, Germany). The luminescence of all samples was quantified using the Synergy HT plate reader (BioTek, Bad Friedrichshall, Germany).
Chen Y., Ye Y., Krauß P.L., Löwe P., Pfeiffenberger M., Damerau A., Ehlers L., Buttgereit T., Hoff P., Buttgereit F, & Gaber T. (2022). Age-related increase of mitochondrial content in human memory CD4+ T cells contributes to ROS-mediated increased expression of proinflammatory cytokines. Frontiers in Immunology, 13, 911050.
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2

Monocyte ATP Content Quantification

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ATP content of monocytes was assessed with the CLS II KIT (Roche, Mannheim, Germany) according to the manufacturer’s instructions. Luminescence of all samples was quantified using the SynergyHT plate-reader (BioTek, Bad Friedrichshall, Germany).
Krauss P.L., Pfeiffenberger M., Damerau A., Buttgereit T., Chen Y., Gaber T, & Buttgereit F. (2021). Production of IL-6 and Phagocytosis Are the Most Resilient Immune Functions in Metabolically Compromised Human Monocytes. Frontiers in Immunology, 12, 730672.
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3

Quantification of Metabolic Activity

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Measurement of glucose and lactate in cell-free supernatant was performed using the biochemistry analyzer Hitachi 917 [17 (link)]. ATP levels were analyzed using CLS II kit (Roche) [15 (link),17 (link)]. Oxygen consumption was measured with a fluorescence-based assay (PreSens, Regensburg, Germany) [17 (link)].
Luger A.L., Lorenz N.I., Urban H., Divé I., Engel A.L., Strassheimer F., Dettmer K., Zeiner P.S., Shaid S., Struve N., Kriegs M., Hofmann U., Oefner P.J., Harter P.N., Steinbach J.P, & Ronellenfitsch M.W. (2020). Activation of Epidermal Growth Factor Receptor Sensitizes Glioblastoma Cells to Hypoxia-Induced Cell Death. Cancers, 12(8), 2144.
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4

Cell Luminescence Quantification

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The samples (50 µL aliquots of each cell suspension) were treated according to manufacturer's instructions (CLS II KIT (Roche Diagnostics GmbH, Mannheim, Germany) and measured against a set of standard dilution rows. Luminescence of all samples was quantified using a luminometer (BD Biosciences, Heidelberg, Germany).
Strehl C., Schellmann S., Maurizi L., Hofmann-Amtenbrink M., Häupl T., Hofmann H., Buttgereit F, & Gaber T. (2016). Effects of PVA-coated nanoparticles on human T helper cell activity. Toxicology letters, 245.
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