Antibodies used are as follow; N-cadherin antibody (ab76011, Abcam, Cambridge, UK), proN-cadherin antibody (GTX101141, GeneTex, San Antonio, TX, USA), GDNF antibody (ab18956, Abcam, Cambridge, MA, USA), N-cadherin (phospho Y860) (ab119752, Abcam), p120-catenin (ab92514, Abcam), β-catenin (GTX22982, Genetex, San Antonio, TX, USA), β-catenin phosphorylated antibodies including phospho Y654 (ab24925, Abcam), phospho Y489 (ab138378, Abcam), phospho Y142 (ab27798, Abcam), Ser33/37/Thr41 (9561, CST, Danvers, MA, USA), nestin (ab22035, Abcam, Cambridge, MA, USA), rabbit anti-CD133 (ab19898, proteintech, America), and anti-Caveolin-1 (SAB4200216, Sigma, Aldrich, USA).
Ab24925
Manufactured by Abcam
Sourced in United States
Ab24925 is a laboratory equipment product manufactured by Abcam. It is a device designed for specific purposes within a laboratory setting. The core function of this product is to facilitate certain laboratory procedures, but no further details on its intended use can be provided in an unbiased and factual manner.
Automatically generated - may contain errors
Lab products found in correlation
Ab76011, by Abcam (1 mentions)
Ab18956, by Abcam (1 mentions)
Ab22035, by Abcam (1 mentions)
Ab27798, by Abcam (1 mentions)
Ab92514, by Abcam (1 mentions)
Ab138378, by Abcam (1 mentions)
Ab119752, by Abcam (1 mentions)
Ab49254, by Abcam (1 mentions)
Ab63568, by Abcam (1 mentions)
Prolong gold antifade containing dapi, by Thermo Fisher Scientific (1 mentions)
Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)
2 protocols using ab24925
1
Comprehensive Antibody Analysis Protocol
2
Immunofluorescence Microscopy of EMT Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Immunofluorescence microscopy using rabbit polyclonal antibodies characterized for human antigens (see table) and phalloidin (Invitrogen) was performed according to previous procedures (McDonald et al., 2013 ). Briefly, cultures were fixed with 4% paraformaldehyde, permeabilized, blocked, and incubated with primary rabbit polyclonal antibodies. Specific primary antibodies used were as follows: 1:1000 dilution of anti‐snail/slug specific for pSer246 (Abcam ab63568), and a 1:500 dilution of anti‐twist (Abcam ab49254) and anti‐β‐catenin (Y654, Abcam ab24925). Alexa Fluor conjugated secondary antibodies were used (Invitrogen), after which cells were counterstained with phalloidin (Invitrogen) and mounted using Prolong Gold antifade containing DAPI (Invitrogen).
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