Octyl α ketoglutarate

Manufactured by Cayman Chemical

Octyl-α-ketoglutarate is a chemical compound used in laboratory settings. It functions as an organic intermediate.

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Lab products found in correlation

Dihydroethidium, by Merck Group (1 mentions) Px 478, by Selleck Chemicals (1 mentions) Nadp nadph kit, by Abcam (1 mentions) Agi 6780, by Selleck Chemicals (1 mentions) β action antibody, by Merck Group (1 mentions) Cocl2, by Merck Group (1 mentions) Propidium iodide, by Merck Group (1 mentions) Fugene, by Promega (1 mentions)

Close spelling variants of this product

Octyl-α-ketoglutarate (octyl-α-KG)

2 protocols using octyl α ketoglutarate

Metabolic Profiling of Cellular Responses

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CoCl₂, dihydroethidium and propidium iodide were from Sigma/Aldrich. Octyl-α-ketoglutarate was from Cayman and PX-478 and AGI-6780 were from Selleck. Antibodies to detect IDH2, HIF1α, LDHA, ALDOA, PDK1, and total ubiquitination and the NADP/NADPH kit were from Abcam and the β-action antibody was from Sigma.

Li J., He Y., Tan Z., Lu J., Li L., Song X., Shi F., Xie L., You S., Luo X., Li N., Li Y., Liu X., Tang M., Weng X., Yi W., Fan J., Zhou J., Qiang G., Qiu S., Wu W., Bode A.M, & Cao Y. (2018). Wild-type IDH2 promotes the Warburg effect and tumor growth through HIF1α in lung cancer. Theranostics, 8(15), 4050-4061.

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SDHD Knockdown in Thyroid Cells

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FTC133 (human follicular thyroid carcinoma) cells were maintained in DMEM media with 10% FBS and penicillin/streptomycin. NthyOri 3.1 (human follicular epithelial thyroid cells) were maintained in RPMI media with 10% FBS and penicillin/streptomycin. Octyl-α-ketoglutarate was purchased from Cayman Chemicals (Ann Arbor, MI). Lentiviral based ShRNA (sequences available on request) for SDHD knockdown was obtained from Sigma Aldrich (St. Louis, MO). Lentiviral supernatant was produced from phoenix cells transfected with Fugene (Promega, Madison WI), packaging mix (Sigma, St. Louis, MO) and viral vector. Stable SDHD-knockdown thyroid cells were generated by puromycin selection after transducing with ShRNA lentivirus.

Ashtekar A., Huk D., Magner A., La Perle K., Zhang X., Piruat J.I., López-Barneo J., Jhiang S.M, & Kirschner L.S. (2017). Sdhd ablation promotes thyroid tumorigenesis by inducing a stem-like phenotype. Endocrine-related cancer, 24(11), 579-591.

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