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Hdl cholesterol

Manufactured by Randox
Sourced in United Kingdom

HDL-cholesterol is a laboratory instrument used to measure the levels of high-density lipoprotein (HDL) cholesterol in blood samples. It provides quantitative analysis of HDL-cholesterol concentrations.

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3 protocols using hdl cholesterol

1

Anthropometric and Metabolic Biomarker Measurement Protocol

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Standardized procedures were used for the measurements of weight and height and body mass index (BMI) was calculated as weight (kg)/height2 (m2).
Blood samples were collected after an overnight fast at the different study point. The serum was separated and immediately stored at −80 °C until posterior analysis. Enzimatic methods were used for the measurement of the levels of cholesterol, triglycerides, high density lipoprotein cholesterol (HDL-cholesterol), and glucose (Randox Laboratories Ltd., Crumlin, UK). The Friedewald formula was used for the calculation of the levels of the low-density lipoprotein (LDL) cholesterol [25 (link)]. Glycosylated hemoglobin (HbA1c) was determined by Dimension Vista autoanalyzer (Siemens Healthcare Diagnostics, Munich, Germany).
The homeostasis model assessment of insulin resistance (HOMA-IR) index was calculated according to the formula: HOMA-IR = fasting plasma insulin (µU/mL) × fasting plasma glucose (mmol/L)/22.5.
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2

Albino Rat Biochemical Assays

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Twenty-four albino male rats (Ratus novergicus) with an average weight of 140 g used for this study were purchased from the Animal Holding Unit of the Department of Biochemistry, University of Ibadan, Nigeria. ATR-MET is a product of First Chemical Co Ltd, Changxing, Zhejiang China. GSH, 1-chloro-2, 4-dinitrobenzene (CDNB), 5′, 5′-dithio-bis-2-nitrobenzoic acid (DTNB), thiobarbituric acid (TBA), epinephrine and hydrogen peroxide were purchased from Sigma Chemical Company (London, UK). ALT, AST, ALP, GGT, urea, creatinine, bilirubin, total cholesterol, HDL-cholesterol, LDL-cholesterol and triglycerides kits were obtained from Randox Laboratories Ltd, Antrim UK. All other chemicals and reagent were of analytical grade and were obtained from British Drug House, Poole, UK.
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3

Moxifloxacin-Induced Oxidative Stress

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Moxifloxacin hydrochloride (Avelox) was a product of Bayer Healthcare Pharmaceutics, Leverkusen, Germany. Glutathione (GSH), 1-chloro-2,4-dinitrobenzene (CDNB), 5′,5′-dithiobis-2-nitrobenzoic acid (DTNB), thiobarbituric acid (TBA), epinephrine, and hydrogen peroxide were purchased from Sigma Chemical Company (London, UK). Assay kits for alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), γ-glutamyl transferase (γ-GT), urea, creatinine, bilirubin, total cholesterol, HDL-cholesterol, LDL-cholesterol, and triglycerides were products of Randox Laboratories Ltd., Antrim, UK. All other chemicals and reagents used in this study were of analytical grade.
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