Hplc mals system

Size exclusion chromatography coupled with multi-angle light scattering (SEC-MALS) experiments were performed using an HPLC-MALS system (Shimadzu) equipped with light scattering detector (mini DAWN TREOS, Wyatt Technology), refractive index detector (Optilab T-rEX, Wyatt Technology) and UV detector (SPD-20A, Shimadzu). Holo-MnmA (100 μl at 2 mg ml⁻¹) was injected on a Superdex 200 10/300 GL increase column (GE Healthcare) equilibrated in 50 mM Tris-HCl (pH 7.5), 200 mM NaCl buffer, in the presence or absence of DTT at a flow rate of 0.5 ml.min⁻¹. Molar masses of proteins were calculated using the ASTRA 6.1 software (Wyatt Technology) using a refractive index increment (dn/dc) value of 0.183 ml g⁻¹.

Size exclusion chromatography coupled with multi-angle light scattering (SEC-MALS) experiments were performed using an HPLC-MALS system (Shimadzu) equipped with light scattering detector (mini DAWN TREOS, Wyatt Technology), refractive index detector (Optilab T-rEX, Wyatt Technology) and UV detector (SPD-20A, Shimadzu). As-purified MmTtuI (100 μl at 2 mg.ml⁻¹) was injected on a Superdex 200 Increase 10/300 GL column (Cytiva) equilibrated in 50 mM HEPES pH 7.5, 200 mM NaCl, at a flow rate of 0.5 ml.min⁻¹. Molar masses of proteins were calculated with the ASTRA 6.1 software (Wyatt Technology) using a refractive index increment (dn/dc) value of  0.183  ml.g⁻¹.