Human hepatocellular carcinoma HepG2 cells and mouse hepatoma Hepa-1c1c7 purchased from the Korean Cell Line Bank (KCLB, Seoul, South Korea) were grown in minimum essential medium (MEM) supplemented with 10% fetal bovine serum (FBS), 100 μg/ml of penicillin-streptomycin, and 25 mM HEPES at 37°C in a humidified environment containing 5% CO2. pHBV-1.2x containing genotype C HBV full-length genome (2.5 μg) was transiently transfected using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA). To normalize the transfection efficacy, pSV-β-Galactosidase (0.25 μg) was co-transfected, and the enzyme assay was accompanied using β-Galactosidase Enzyme Assay System with Reporter Lysis buffer (Promega, Madison, WI, USA), following the manufacturers' protocol.
Hepa 1c1c7
Manufactured by Korean Cell Line Bank
Sourced in United States
The Hepa-1c1c7 is a cell line derived from mouse hepatoma cells. It is a well-characterized and widely used in vitro model for studying various cellular processes and pathways. The core function of the Hepa-1c1c7 cell line is to serve as a research tool for investigating liver-related biological and biochemical mechanisms.
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3 protocols using hepa 1c1c7
1
Transient HBV Transfection in Hepatoma Cells
2
Induction of Inflammatory Response in Hepatocytes
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The murine hepatoma cell line (Hepa-1c1c7) and human fibroblast cell line (HFF) were obtained from the Korean Cell Line Bank (Seoul, Korea). The retrovirus packaging cell line (Phoenix) was purchased from Cell Biolabs (San Diego, CA, USA). Hepa-1c1c7, HFF, and Phoenix cells were cultured in high-glucose Dulbecco’s modified Eagle medium (DMEM; WELGENE Inc., Gyeongsan, Korea) containing 10% fetal bovine serum (FBS; WELGENE) and 1% antibiotic–antimycotic (AA) solution (WELGENE) at 37°C in a 5% CO2 incubator. To induce an inflammatory environment in hepatocytes, Hepa-1c1c7 vector cells and Hepa-1c1c7 TgIST cells were seeded in a 6-well plate at 3×105 cells/well and incubated for 24 h. The cells were stimulated with 100 ng/ml of IFN-γ/TNF-α for 24 h before conducting the experiment.
3
Murine Cell Line Culture Conditions
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Murine-derived macrophage (RAW 264.7) and hepatoma (hepa-1c1c7) cells were obtained from the Korean Cell Line Bank (KCLB; Seoul, Korea) and were cultured in Dulbecco’s Modified Eagle Medium (DMEM: HyClone, Logan, UT, USA) and Minimum Essential Medium (MEM; HyClone), respectively. These cells were cultured at 37 °C in a medium supplemented with 10% fetal bovine serum (FBS; HyClone), 100 U/mL penicillin and 100 μg/mL streptomycin (HyClone) in a humidified atmosphere containing 95% air and 5% CO2.
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