Cells in BALF were stained with fluorescently labeled antibodies: CD45-Alexa Fluor 700 (30-F11), CD11b-PE-Cy7 (M1/70), Ly6G-PerCP-Cy5.5 (1A8), CD11c-BV605 (N418), CD45-Alexa Fluor 700 (30-F11), BV510-CD103 (2E7), SiglecF-Alexa Fluor 647 (E50-2440; BD Biosciences), MHCII-APC-Cy7 (M5/114.15.2), Ly6C-PE-Texas red (AL-2; BD Biosciences), and DAPI to assess cell viability. Uniform dyed microspheres, Dragon Green (Bangs Laboratories, Inc.), were used for cell counting. Antibodies were purchased from BioLegend. Data were collected by flow cytometry using an LSRFortessa X-20 cytometer (Becton Dickinson), and results were analyzed using FlowJo v.10.
Cd45 alexa fluor 700 30 f11
Manufactured by BD
CD45-Alexa Fluor 700 (30-F11) is a fluorescently labeled antibody that targets the CD45 cell surface antigen. It is designed for use in flow cytometry applications.
Automatically generated - may contain errors
1 protocol using cd45 alexa fluor 700 30 f11
1
Multicolor Flow Cytometry Analysis of BALF
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