Mouse anti γ tubulin t6557

FGF-SAP (Advanced Targeting Systems, Carlsbad, CA, USA) is a chemical conjugate of FGF2 and saporin.
Unconjugated saporin, SAP (Advanced Targeting Systems, Carlsbad, CA, USA), served as control for the targeted ligand toxin. There are approximately 1.5 molecules of saporin conjugated per molecule of FGF2, which was accounted for when treating the cells with saporin versus FGF-SAP.
The plasmid pDEST15-GST-FGF2 was a generous gift from Dr. Malgorzata Zakrzewska (Department of Protein Engineering, Faculty of Biotechnology, University of Wroclaw, Poland) and recombinant GST-FGF2 was prepared as previously described [35 (link)].
The following antibodies were used: mouse anti-γ-tubulin (T6557) from Sigma-Aldrich (St. Louis, MO, USA), mouse anti-GST (Sc-138) and rabbit anti-GST (Sc-459) from Santa Cruz Biotechnology (Dallas, TX, USA), mouse anti-Lamp1 (1D4B) from Developmental Studies Hybridoma Bank. HRP-conjugated secondary antibodies were from Dako (Glostrup, Denmark). Fluorescently labeled secondary antibodies were from Jackson ImmunoResearch Laboratories (Cambridgeshire, UK).
Hoechst 33,258 and heparin were from Sigma Aldrich (St. Louis, MO, USA). cOmplete EDTA-free protease inhibitor cocktail was from Roche (Basel, Switzerland).

Mouse anti-Plk4 antibody has been described previously (Cizmecioglu et al., 2010 (link)) and used at a final concentration of 1 µg/ml. Mouse anti-Flag M2 (F3165), mouse anti-α-tubulin (T5168) and mouse anti-γ-tubulin (T6557) were from Sigma. Mouse anti-Myc (9E10), mouse anti-Plk1 (F-8), and mouse anti-cyclin E (HE12) were obtained from Santa Cruz Biotechnology. Mouse anti-actin (JLA20) was from Calbiochem, mouse anti-His from Qiagen, rabbit anti-GFP (NB600-303) from Novus, rabbit anti-CP110 (A301-343A) and rabbit anti-STIL (A302-442A) for western blotting from Bethyl, rabbit anti-STIL (ab89314) for immunofluorescence and rabbit anti-pericentrin (ab4448) from Abcam. Rabbit anti-cyclin B1 has been described previously (Hoffmann et al., 1993 (link)). Secondary antibodies for western blotting were peroxidase-conjugated donkey anti-rabbit (Jackson Laboratories) and goat anti-mouse (Novus). Secondary antibodies for immunofluorescence were goat anti-mouse IgG and goat anti-rabbit IgG coupled to Alexa Fluor 488 or Alexa Fluor 594 (Molecular Probes).