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Sorbitol is a sugar alcohol commonly used as a sweetener in various food and pharmaceutical products. It is a white, crystalline powder that is naturally found in many fruits and berries. Sorbitol has a sweet taste and is often used as a replacement for sucrose in low-calorie and diabetic-friendly formulations due to its lower glycemic index. It is a versatile ingredient with applications in the production of candies, chewing gum, and other confectionery items.

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4 protocols using sorbitol

1

Formulation of Cellulose-Based Hydrogels

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Commercially available food-grade hydroxypropyl methylcellulose (HPMC) (HT-E15), XG (Mw = 500,000–600,000), GG (Mw = 400,000–600,000), potassium citrate, and calcium chloride were purchased from Shandong Rutocel Co., Ltd. (Taian, China). Sodium alginate (Mw = 98,000; G/M = 1), fructose (Mw = 180.15) and sorbitol were provided by Shanghai Macklin Biochemical Technology Co., Ltd. (Shanghai, China). Glycerol, beeswax, and PEG were supplied by Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).
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2

Cultivation and Harvesting of Gluconobacter oxydans NL71

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Gluconobacter oxydans NL71 strain, derived from ATCC621, was stored in sorbitol-agar medium containing 50 g/L sorbitol, 5 g/L yeast extract, and 15 g/L agar under 4 °C. G. oxydans NL71 inoculum was cultivated in a 250-mL Erlenmeyer flask containing 50 mL of culture medium consisting of 100 g/L sorbitol and 10 g/L yeast extract, then incubated for 24–36 h at 30 °C and 220 rpm. The proliferative cells were harvested by refrigerated centrifugation (Avanti J26 XP, Beckman Coulter) at 6000 rpm for 5 to 10 min [31 , 32 (link)].
Biomass-based butanol, BA, sorbitol and sorbose were purchased from Macklin, while yeast extract was obtained from Sigma. All other chemicals such as nutrients and NaOH, were of analytical grade and were commercially available.
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3

Formulation and Analysis of Fluopyram

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Fluopyram (97.5% purity) was purchased from Bayer (China) Co., Ltd. (Leverkusen, Germany). 1-Dodecanesulfonic acid sodium salt (SDS), sodium dodecyl benzene sulfonate (SDBS), sodium lignosulfonate (SL, methoxy group: 5~7%, carbon: 45~47%), hydroxypropyl methyl cellulose (HPMC, hydroxylpropoxyl: 4~12%, methoxy: 19~24%, 100 mPa.s), sodium salt of polynaphthalene sulphonic acid (NNO), white carbon, diatomite, glucose, sucrose, mannitol, anhydrous d-trehalose, and sorbitol were obtained from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Pluronic F127 was obtained from Sigma-Aldrich (Shanghai, China). Polycarboxylate (TERSPERSE 2700) was provided by Huntsman Technology Corporation (USA). Morwet D425 and Morwet EFW were provided by Nanjing Jierun Technology Co., Ltd. (Nanjing, China). LC/MS-grade and HPLC-grade methanol and acetonitrile were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Ultrapure water was used in all experiments. Qiagen RLT Lysis solution was purchased from Beijing Huaxia Ocean Technology Co., Ltd. (Beijing, China).
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4

Comprehensive Pesticide Residue Analysis

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A total of 33 pesticide reference standards with initial concentrations of 100 µg/mL and purities of 98.0-99.9% were supplied by the Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, China (see Table A1 in the Appendix). These pesticides were selected based upon their common application on tea plants and could be analyzed through GC-MS/MS. Primary secondary amine (PSA), graphitized carbon black (GCB), and C18 sorbents were purchased from Agela Technologies (Tianjin, China). HPLC grade acetonitrile and toluene were purchased from Sigma-Aldrich (Merck, Germany). Analytical grade sodium chloride (NaCl) and anhydrous magnesium sulfate (MgSO4) were purchased from China National Medicines Corporation Ltd. (Shanghai, China). Heptachlor epoxide with a purity ≥ 98.0% was used as an internal standard (IS) for GC-MS/MS analysis. The stock IS solution was prepared in acetonitrile to a concentration of 4 µg/mL and preserved at -20 °C until used. An analyte protectant (AP) solution was also prepared in acetonitrile, comprising 10 mg/mL of sorbitol and 20 mg/mL of L-gulonic acid-γ-lactone (both ≥ 98.0% purity; Macklin, Shanghai, China).
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