Pe anti mouse cd86 159204

The DMEM, FBS and penicillin-streptomycin were acquired from Gibco (Grand Island, USA). Additionally, the trypsin-EDTA was obtained from Sigma-Aldrich (St Louis, USA). The primary antibodies against CD68 (ab283654), CD86 (ab239075), iNOS2 (ab283655), ARG (ab264066) and AGEs have been procured from Abcam (Cambridge, UK). PE anti-mouse CD86 (159204) and FITC anti-mouse CD206 (141703) have been collected from BioLegend (San Diego, USA). Alpha-smooth muscle actin (α-SMA) (BM0002) has been obtained from Boster Biological Technology (Wuhan, China). BCL-2 (AF6139), BAX (AF0120) and Caspase 9 (AF7022) antibodies were gathered from Affinity (Georgia, USA) and C-caspase 3 antibody has been procured from Bioss (Wuhan, China). Masson’s staining kit (G1340) was collected from Solarbio (Beijing, China).

Melatonin, fetal bovine serum (FBS) and trypsin-EDTA were purchased from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM) and penicillin–streptomycin were purchased from Gibco Life Technologies (Grand Island, NY, USA). The antibodies of advanced glycosylation end products (AGEs) of bovine serum albumin (AGEs-BSA), primary antibodies against CD68 (ab283654), CD86 (ab239075), nitric oxide synthase 2 (iNOS2, ab283655) and ARG (arginase Type II, ab264066) were purchased from Abcam (Cambridge, UK). PE anti-mouse CD86 (159204) and FITC anti-mouse CD206 (141703) were purchased from BioLegend (San Diego, CA, USA), and alpha-smooth muscle actin (α-SMA) (BM0002) was purchased from Boster Biological Technology (Wuhan, China). Masson’s Staining Kit (G1340) was purchased from Solarbio (Beijing, China).