Gena934

Manufactured by GE Healthcare

The GENA934 is a lab equipment product by GE Healthcare. It is designed to perform essential functions in a laboratory setting. The core function of this product is to provide reliable and consistent performance for laboratory processes. Further details on the specific intended use of this product are not available.

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Lab products found in correlation

Na931, by GE Healthcare (1 mentions) Ab8224, by Abcam (1 mentions) Ab6161, by Abcam (1 mentions) Ab113687, by Abcam (1 mentions) Ab1227, by Abcam (1 mentions) Ab97057, by Abcam (1 mentions) Ab24609, by Abcam (1 mentions) M4439, by Merck Group (1 mentions) E cadherin, by BD (1 mentions) Phospho β catenin, by Cell Signaling Technology (1 mentions) Phospho gsk3β, by Cell Signaling Technology (1 mentions) C myc, by Abcam (1 mentions) E cadherin, by Cell Signaling Technology (1 mentions) β catenin, by BD (1 mentions) β actin c4, by Santa Cruz Biotechnology (1 mentions) Lamin b c 20, by Santa Cruz Biotechnology (1 mentions) Zo 1 d6l1e, by Cell Signaling Technology (1 mentions) Gena931, by GE Healthcare (1 mentions) Phospho cofilin, by Cell Signaling Technology (1 mentions) Gapdh, by GeneTex (1 mentions)

2 protocols using gena934

Western Blot Protocol for Diverse Protein Detection

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Western blots were performed as described^{12 (link)}, using the following primary antibodies: mouse monoclonal anti-beta actin (Abcam, ab8224, RRID: AB_449644, 1:1000 dilution), rat monoclonal anti-tubulin (Abcam, ab6161, RRID: AB_305329, 1:4000 dilution), mouse monoclonal anti-Pgk1 (Abcam, ab113687, RRID: AB_10861977, 1:5000 dilution), rabbit polyclonal anti-biotin (Abcam, ab1227, RRID: AB_298990, 1:3000 dilution), mouse monoclonal anti-c-Myc (clone 9E10, Sigma-Aldrich, M4439, RRID: AB_439694, 1:4000 dilution), mouse monoclonal anti-nuclear pore complex proteins (Mab414, Abcam, ab24609, RRID: AB_448181, 1:5000 dilution). Secondary antibodies used were goat anti-rat IgG (Abcam, ab97057, RRID: AB_10680316, 1:10,000 dilution), donkey anti-rabbit IgG (GE Healthcare, GENA934, RRID: AB_2722659, 1:10,000 dilution) and sheep anti-mouse IgG (GE Healthcare, NA931, RRID: AB_772210, 1:10,000 dilution). See “Quantification and statistical analysis” for a detailed description of western blot quantifications.

Fontana G.A., Hess D., Reinert J.K., Mattarocci S., Falquet B., Klein D., Shore D., Thomä N.H, & Rass U. (2019). Rif1 S-acylation mediates DNA double-strand break repair at the inner nuclear membrane. Nature Communications, 10, 2535.

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Immunoblotting of Epithelial-Mesenchymal Transition Markers

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Antibodies recognizing E-cadherin (24E10) (3195), Snail (C15D3) (3879), ROCK1 (C8F7) (4035), ROCK2 (D1B1) (9029), cofilin (D3F9) (5175), phospho-cofilin (Ser3) (77G2) (3313), phospho-β-catenin (S33/37/41) (9561), phospho-GSK-3β (Ser9) (D85E12) (5558), GSK-3β (D5C5Z) (12456), Axin2 (76G6) (2151), Sox-9 (D8G8H) (82630) ZO-1 (D6L1E) (13663), and DKK-1 (D5V6L) (48367) were obtained from Cell Signaling Technology (Danvers, MA). Additional antibodies used were E-cadherin (BD Transduction Laboratories, 610181), β-catenin (BD Transduction Laboratories, 610153), c-myc (Abcam, Cambridge, United Kingdom; ab32072), GPCR GPR49 (Lgr5) (Abcam, ab75850), GAPDH (GeneTex, Irvine, CA; GTX627408), Lamin B (C-20) (Santa Cruz Biotechnology, Santa Cruz, CA; sc-6216), and β-actin (C4) (Santa Cruz Biotechnology, sc-47778). Secondary HRP-conjugated antibodies used were rabbit immunoglobulin G HRP-linked (GE Healthcare, Chicago, IL; GENA934) and mouse immunoglobulin G HRP-linked (GE Healthcare, GENA931).

Dunbar K., Valanciute A., Lima A.C., Vinuela P.F., Jamieson T., Rajasekaran V., Blackmur J., Ochocka-Fox A.M., Guazzelli A., Cammareri P., Arends M.J., Sansom O.J., Myant K.B., Farrington S.M., Dunlop M.G, & Din F.V. (2020). Aspirin Rescues Wnt-Driven Stem-like Phenotype in Human Intestinal Organoids and Increases the Wnt Antagonist Dickkopf-1. Cellular and Molecular Gastroenterology and Hepatology, 11(2), 465-489.

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