A-431 (ATCC CRL-1555) and HeLa epithelial cells (ATCC CCL-2) were cultured, at 37°C and in 5% CO2, in DMEM supplemented with 15% (vol/vol) fetal bovine serum (FBS, Life Technologies) and 1 IU penicillin/streptomycin ml−1 (Lonza). Cells were cultured, at 37°C and 5% CO2, in 24-well tissue culture plates (Iwaki) until they formed a monolayer. Before the adhesion assays, the cells were washed twice with 500 μl of sterile phosphate -buffered saline (PBS) to remove non adherent cells and culture media.
24 well tissue culture plates
Manufactured by Iwaki
Sourced in United States
24-well tissue culture plates are a commonly used laboratory equipment for cell culture applications. These plates provide a standardized format with 24 individual wells, each designed to accommodate a specific volume of cell culture media and cells. The plates are constructed from high-quality, durable materials that are suitable for a variety of cell culture experiments and techniques.
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Lab products found in correlation
Penicillin streptomycin, by Lonza (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Biowest (1 mentions)
Phorbol myristate acetate (pma), by Fujifilm (1 mentions)
Il 13, by BioLegend (1 mentions)
Rpmi 1640 with l glutamine, by Fujifilm (1 mentions)
Thp 1, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Isogen with a spin column, by Nippon Gene (1 mentions)
Recombinant human ifng, by BioLegend (1 mentions)
Revertra ace qpcr rt master mix, by Toyobo (1 mentions)
Thunderbird sybr qpcr mix, by Toyobo (1 mentions)
Interleukin 4 (il 4), by BioLegend (1 mentions)
2 protocols using 24 well tissue culture plates
1
Culturing A-431 and HeLa cells
2
Macrophage Differentiation and Activation
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Spectra/Por ® 6 Dialysis Membrane (MWCO: 10 kDa, Spectrum Laboratories, CA, USA) was purchased and used as cellulose. We obtained 6-nylon (KOKUGO, Tokyo, Japan), polyethylene terephthalate film (PET, Toray Industry, Tokyo, Japan), polytetrafluoroethylene (PTFE, Fron Industry, Tokyo, Japan), polymethylmethacrylate (PMMA, MW 350000, Sigma, St. Louis, MO, USA) and 24-well tissue culture plates (TCPS, IWAKI, Shizuoka, Japan). CELLSPIN and 100 ml spinner flasks were purchased from Pfeiffer Electronic Engineering GmbH (Lahnau, Germany). PMA, RPMI-1640 with L-glutamine and lipopolysaccharide from Escherichia coli O26 (LPS) were purchased from FUJIFILM Wako (Osaka, Japan). FBS was purchased from Biowest (Nuaillé, France). Recombinant human IFN-g, IL-4 and IL-13 were obtained from BioLegend (CA, USA). ISO-GEN with a spin column was purchased from NipponGene (Tokyo, Japan). The ReverTra Ace qPCR RT Master Mix and THUNDERBIRD™ SYBR ® qPCR Mix were obtained from Toyobo (Osaka, Japan). THP-1 were purchased from JCRB Cell Bank (Osaka, Japan).
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