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Lenti sestrin2

Manufactured by RiboBio
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Sourced in China

Lenti-Sestrin2 is a lentiviral vector that expresses the Sestrin2 protein. Sestrin2 is a cellular stress sensor that regulates metabolic homeostasis. The Lenti-Sestrin2 product provides a tool for studying the role of Sestrin2 in cellular processes.

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Lab products found in correlation

Lipofectamine 2000, by RiboBio (2 mentions) Lipofectamine 2000 reagent, by Thermo Fisher Scientific (2 mentions) Sisestrin2, by RiboBio (2 mentions)

2 protocols using lenti sestrin2

1

Sestrin2 Regulation by miR-182

Check if the same lab product or an alternative is used in the 5 most similar protocols
Gene regulation of Sestrin2 was based on the lentivirus system. The ectopic expression clones of Sestrin2 were constructed based on the pcDH-puro plasmids (Lenti-Sestrin2), and small interfering RNA of Sestrin2 (siSestrin2) was obtained from Guangzhou RiboBio Co. Ltd. (Guangzhou, China). All plasmids were transferred into 293 cells using Lipofectamine-2000 reagent (Invitrogen) according to the manufacturer's protocol for 48 h, and the medium was harvested and added to the ASMCs to study the changes in gene expression.
MiR- 182 mimics, miR- 182 inhibitor, and their control small RNAs were obtained from Guangzhou RiboBio Co. Ltd. and transfected into cells using Lipofectamine-2000 for 48 h based on the manufacturer's protocol.
Xiao Y., Zhu H., Lei J., Xie J., Wu K., Gu W., Ma J., wei D., Shu Z, & Zhao L. (2021). MiR-182/Sestrin2 affects the function of asthmatic airway smooth muscle cells by the AMPK/mTOR pathway. Journal of Translational Internal Medicine, 11(3), 282-293.
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2

Sestrin2 Regulation by miR-182

Check if the same lab product or an alternative is used in the 5 most similar protocols
Gene regulation of Sestrin2 was based on the lentivirus system. The ectopic expression clones of Sestrin2 were constructed based on the pcDH-puro plasmids (Lenti-Sestrin2), and small interfering RNA of Sestrin2 (siSestrin2) was obtained from Guangzhou RiboBio Co. Ltd. (Guangzhou, China). All plasmids were transferred into 293 cells using Lipofectamine-2000 reagent (Invitrogen) according to the manufacturer's protocol for 48 h, and the medium was harvested and added to the ASMCs to study the changes in gene expression.
MiR- 182 mimics, miR- 182 inhibitor, and their control small RNAs were obtained from Guangzhou RiboBio Co. Ltd. and transfected into cells using Lipofectamine-2000 for 48 h based on the manufacturer's protocol.
Xiao Y., Zhu H., Lei J., Xie J., Wu K., Gu W., Ma J., wei D., Shu Z, & Zhao L. (2021). MiR-182/Sestrin2 affects the function of asthmatic airway smooth muscle cells by the AMPK/mTOR pathway. Journal of Translational Internal Medicine, 11(3), 282-293.
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+ Expand

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