N-acetylcysteine (NAC; A7250), dimethyl sulfoxide, 4′,6-diamidino-2-phenylindole, and anti-β-actin (A1978) were from Sigma-Aldrich (St. Louis, MO). Recombinant human prorenin was from Abcam (ab93266; Cambridge, MA) and MCC950 (sc-505904) was from Santa Cruz Biotechnology (Santa Cruz, CA). The ROS fluorescent probe-DCFH-DA kit (S0033) and caspase 1 activity assay kit (C1101) were from the Beyotime Institute of Biotechnology (Nanjing, China). The following antibodies were used: sheep monoclonal antiserum against prorenin/renin (GTX7967, Gene Tex, San Antonio, TX); rabbit polyclonal antiserum against PRR (bs-7691R, Bioss, Beijing, China); rabbit polyclonal antiserum against caspase-1 (D7F10); rabbit anti-ASC monoclonal antibody (#13833), anti-CD86 (#91882), and anti-CD206 (#91992) were from Cell Signaling Technology (Beverly, MA); mouse monoclonal antiserum against CD11b/c (OX42, ab1211), mouse monoclonal to IL-6 (ab9324), iNOS (ab15323), Arg (ab60176), Iba-1 (ab153696), GFAP (ab7260), PGP 9.5 (ab10410), and rabbit polyclonal antiserum against NLRP3 (ab214185) were from Abcam. Donkey anti-sheep IgG H&L Alexa Fluor 555 (ab150178), goat anti-rabbit IgG H&L Alexa Fluor 594 (ab150080), and goat anti-mouse IgG H&L Alexa Fluor 488 (ab150117) were from Abcam. MCC950 was from Adipogen Corp. (San Diego, CA). PLX5622 was from Plexxikon (Berkeley, CA).
Mcc950 sc 505904
Manufactured by Santa Cruz Biotechnology
Sourced in Israel
MCC950 (sc-505904) is a laboratory reagent product offered by Santa Cruz Biotechnology. It is a small molecule that functions as an inhibitor. No further details about its specific application or intended use can be provided in an unbiased and factual manner.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd206, by Cell Signaling Technology (1 mentions)
Goat anti mouse igg h l alexa fluor 488 ab150117, by Abcam (1 mentions)
Ab153696, by Abcam (1 mentions)
Ab214185, by Abcam (1 mentions)
Ab60176, by Abcam (1 mentions)
Ab9324, by Abcam (1 mentions)
Ab7260, by Abcam (1 mentions)
Anti β actin a1978, by Merck Group (1 mentions)
Plx5622, by Plexxikon (1 mentions)
Mcc950, by Adipogen (1 mentions)
Cell mitochondria isolation kit, by Beyotime (1 mentions)
Z yvad fmk, by Merck Group (1 mentions)
Cell counting kit 8 (cck8), by Beyotime (1 mentions)
Oxygen consumption rate assay kit, by Abcam (1 mentions)
Mitosox red mitochondrial superoxide indicator, by Thermo Fisher Scientific (1 mentions)
Mitochondria storage buffer, by Beyotime (1 mentions)
2 protocols using mcc950 sc 505904
1
Prorenin Receptor Activation and Inflammasome
2
Mitochondrial Dysfunction and Inflammasome Regulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Neu-p11 (Piromelatine) was purchased from Neurim Pharmaceuticals Ltd. (Tel-Aviv, Israel); MCC950 (sc-505904) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, United States). Z-YVAD-FMK (235429) was purchased from Sigma-Aldrich (Sigma, St Louis, MO, USA). GKT137831 (B4763) was purchased from ApexBio Technology (ApexBio, Houston, Texas, USA). Oxygen Consumption Rate Assay Kit (ab197243) was purchased from Abcam (Cambridge, UK). Mito-SOX Red Mitochondrial Superoxide Indicator) (M36008) was purchased from Invitrogen (Carlsbad, CA, USA). Cell Counting Kit-8 (C0037), LDH Cytotoxicity Assay Kit (C0016), Mitochondrial membrane potential assay kit with JC-1 (C2006), Caspase 1 activity assay kit (C1101), Cell Mitochondria Isolation Kit (C3601) and Mitochondria Storage Buffer (C3609) were purchased from Beyotime (Nanjing, PRC). The antibodies used in this study are: β-actin (ab179467), NOX4 (ab133303), NLRP3 (ab232401), ASC (ab155970), pro-Caspase-1 (ab179515), PARP (ab74290), MnSOD (ab13533), Sirt3 (ab223531), Tfam (ab131607), COX IV (ab33985), Donkey Anti-Goat IgG H&L(Cy5) (ab6566), Donkey Anti-Rabbit IgG H&L(HRP) (ab6802) were purchased from Abcam (Cambridge, UK). Cleaved-Caspase-1(D57A2) was purchased from Cell Signaling Technology (Beverly, MA, USA); pro-IL-1β (AF-401) was purchased from R&D Systems (Minneapolis, USA).
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