HIF-1 and PHD activities were measured using a dual luciferase kit from Promega with HRE-luciferase (Plasmid #26731, Addgene) as reporter of HIF-1 activity and ODD-luciferase (Plasmid #18965, Addgene) as reporter of PHD activity. pRL Renilla luciferase (Promega) was used as internal control. Cells were co-transfected using the TransIT-2020 transfection reagent (Mirus Bio).
Odd luciferase
Manufactured by Addgene
The ODD-luciferase is a reporter gene construct that expresses a fusion protein of the oxygen-dependent degradation (ODD) domain of the hypoxia-inducible factor-1 alpha (HIF-1α) and the firefly luciferase enzyme. The ODD domain targets the luciferase for degradation under normoxic conditions, leading to low basal luciferase activity. Under hypoxic conditions, the ODD domain is stabilized, resulting in increased luciferase activity that can be detected and quantified.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using odd luciferase
1
Quantifying HIF-1 and PHD Activity
2
Quantifying HIF-1 and PHD Activity
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HIF-1 and PHD activities were measured using a dual luciferase kit from Promega with HRE-luciferase (Plasmid #26731, Addgene) as reporter of HIF-1 activity and ODD-luciferase (Plasmid #18965, Addgene) as reporter of PHD activity. pRL Renilla luciferase (Promega) was used as internal control. Cells were co-transfected using the TransIT-2020 transfection reagent (Mirus Bio).
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