Rabbit anti phosphogsk3β ser9 d85e12

Manufactured by Cell Signaling Technology

Rabbit anti-PhosphoGSK3β (Ser9) (D85E12) is a primary antibody that detects the phosphorylation of GSK3β at serine 9. It is commonly used in western blotting applications to analyze the activation state of the GSK3β protein.

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Lab products found in correlation

Rabbit anti ha, by Merck Group (1 mentions) Rabbit anti rac1b, by Merck Group (1 mentions) Mouse anti srpk1 and mouse anti β catenin, by BD (1 mentions) Mouse anti pcna, by Merck Group (1 mentions) Ab108387, by Abcam (1 mentions) Mouse anti p44 42 mapk erk1 2 l34f12, by Cell Signaling Technology (1 mentions) Mouse anti gsk3β clone 3d10, by Cell Signaling Technology (1 mentions) Rabbit anti phospho sfk y416 clone 100f9, by Cell Signaling Technology (1 mentions) Rabbit anti fyn clone epr5500, by Merck Group (1 mentions) Mouse anti actin clone c4, by Merck Group (1 mentions)

2 protocols using rabbit anti phosphogsk3β ser9 d85e12

Antibody Characterization for Western Blot Analysis

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Samples were prepared and detected as described [49 (link)]. The antibodies used for Western blots were: mouse anti-α-tubulin (clone B-5-1-2), mouse anti-SR proteins (clone 1H4), and rabbit anti-HA from Sigma-Aldrich; rabbit anti-RAC1B from Millipore; mouse anti-PCNA from MerckBiosciences; mouse anti-SRPK1 and mouse anti-β-catenin from BD Transduction Laboratories; rabbit anti-AKT, rabbit anti-GSK3β (27C10) and rabbit anti-PhosphoGSK3β (Ser9) (D85E12) from Cell Signalling; mouse anti-p-GSK-3α/β (6D3) (sc-81496), mouse anti-SF2/ASF (96) (sc-33652) (SRSF1) and anti-Histone H2b (sc-10808) from Santa Cruz Biotechnology and sheep anti-WNK1 from Dundee University. For the detection of phospho-SRSF1, total SRSF1 was first immunoprecipitated with the specific anti-SRSF1 antibody (sc-33652) and this enriched fraction then probed with the-SR phosphoepitope antibody (1H4). For densitometric analysis of band intensities, X-ray films from at least three independent experiments were digitalized and analyzed using ImageJ software (NIH).

Gonçalves V., Henriques A.F., Matos P, & Jordan P. (2020). Ibuprofen disrupts a WNK1/GSK3β/SRPK1 protein complex required for expression of tumor-related splicing variant RAC1B in colorectal cells. Oncotarget, 11(47), 4421-4437.

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Molecular Mechanisms of Tau Hyperphosphorylation

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Unless indicated, all chemicals were purchased from Sigma/Merck Millipore. Antibodies used for immunoblotting included: mouse anti‐demethylated PP2Ac (clone 1D6, Merck Millipore); mouse anti‐PP2Ac_α/β (clone 46, BD Transduction); rabbit anti‐phospho‐p44/42 mitogen activated protein kinase (MAPK) (#9101, Cell Signaling Technology); mouse anti‐p44/42 MAPK (ERK1/2) (L34F12, Cell Signaling Technology); rabbit anti‐phospho‐GSK3β (Ser9) (D85E12, Cell Signaling Technology); mouse anti‐GSK3β (clone 3D10, Cell Signaling Technology); rabbit anti‐Tau (T‐1308‐1, rPeptide); mouse anti‐pS202‐Tau (CP13) [41 (link)] and anti‐pSer396/Ser404 (PHF‐1) tau (kindly donated by Peter Davies); mouse anti‐pS422‐tau (44‐764G, ThermoFisher); mouse anti‐human Tau‐13 (ab19030, Abcam); rabbit anti‐Fyn (clone EPR5500, Merck Millipore); mouse anti‐Fyn clone 25 (BD Transduction Laboratories); rabbit anti‐phospho‐SFK (Y416) (clone 100F9, Cell Signaling Technology); mouse anti‐actin (clone C4, Merck Millipore); and mouse anti‐glyceraldehyde 3‐phosphate dehydrogenase (clone 6C5, Merck Millipore). Mouse anti‐Fyn (clone 15, Santa Cruz), rabbit anti‐pS202‐Tau (ab108387, Abcam) and PHF‐1 antibodies were used for immunostaining.

van Hummel A., Taleski G., Sontag J., Feiten A.F., Ke Y.D., Ittner L.M, & Sontag E. (2023). Methyl donor supplementation reduces phospho‐Tau, Fyn and demethylated protein phosphatase 2A levels and mitigates learning and motor deficits in a mouse model of tauopathy. Neuropathology and Applied Neurobiology, 49(4), e12931.

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