As 220 r2

Apparatus. In this study a fluorescence spectrophotometer, F-7000 (Hitachi, Japan) equipped with 1.0 cm quartz cell, a thermostatic water bath (Unitronic Orbital, P Spectra, Spain), an analytical balance (AS 220.R2 Shimadzu, Japan), a sonicator (UltrasonsMedi II), pH meter (Orion Star A111) were also used for the analysis.
Drugs and chemicals. Standard secnidazole was gift from ACI Pharmaceuticals Ltd., Dhaka, Bangladesh. Potassium dihydrogen orthophosphate, potassium phosphate dibasic and BSA were of analytical grade and purchased from the local market. The BSA solution was prepared in phosphate buffer (pH 7.4). All metal salts were collected from department of Pharmaceutical Chemistry, university of Dhaka, Bangladesh. (Saha et al., 2013) pH 7.4. About 65 ml of 0.01M KH 2 PO 4 was mixed with 235 ml of 0.01M K 2 HPO 4 and diluted to 1000 ml with DM water.

A digitalized melting-point machine (WRS-1B), an electronic balance (AS 220.R2 Shimadzu, Japan), sonicator (UltrasonsMediIi), pH meter (Orion Star A111), thermostatic water bath (Unitronic Orbital, P Spectra, Spain), TGA 50H (Shimadzu, Japan), JEOL/EO-JSM-6490 equipment, RIGAKU Ultima IV Xray Diffractometer, and so on, were utilized for the in-vitro analysis of drug-drug interaction. On the other hand, the F-7000 Spectrophotometer (Hitachi, Japan) was used for the synchronous fluorescence spectroscopic analysis, and various extensive computer-based software like PyRx, PyMol, Discovery Studio 4.5, etc., were run for molecular docking intended for receptor-based analysis.