H1299 was purchased from American Type Culture Collection (ATCC) and cultured in RPMI 1640 (Invitrogen) with 5% FBS (Hyclone), at 37°C in 10% CO2. YM155 (S1130) and BI6727 (S2235) were purchased from Selleck Chemicals. L-mimosine, nocodazole and 5-BrdU were products of Sigma-Aldrich. All antibodies for Western Blot analysis of cell cycle proteins were from Cell Signaling Technology including p21, p27, Cyclin D1, Cdt1, Cyclin A1, Cyclin E1, PCNA and Cyclin B1. Actin B antibody was purchased from Sigma. Anti-BrdU-APC was from eBioscience. HU331 was purchased from Abcam (ab120922).
Hu331
Manufactured by Abcam
HU331 is a chemical compound that acts as a cannabinoid receptor agonist. It is used in research applications.
Automatically generated - may contain errors
Lab products found in correlation
Nocodazole, by Merck Group (1 mentions)
Anti brdu apc, by Thermo Fisher Scientific (1 mentions)
L mimosine, by Merck Group (1 mentions)
Cyclin d1, by Cell Signaling Technology (1 mentions)
5 brdu, by Merck Group (1 mentions)
Ym155, by Selleck Chemicals (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Bi6727, by Selleck Chemicals (1 mentions)
H1299, by American Type Culture Collection (1 mentions)
Alpha ftir spectrometer, by Bruker (1 mentions)
2 protocols using hu331
1
Investigating Cell Cycle Regulation
2
Characterization of Compound HU-331
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Reactants and reagents were used as received.
Commercial HU-331 was purchased from Abcam.
Compound homogeneity was monitored by ascending thin-layer chromatography, performed on Merck 0.2 mm aluminium-backed silica gel 60 F254 plates and visualised using ultraviolet irradiation (254 nm) or by using aqueous alkaline potassium permanganate. Flash column chromatography was performed using Merck silica gel 60 of particle size 33-70 mm. Evaporation refers to the removal of solvent under reduced pressure. Melting points were determined using an Optimet apparatus and are uncorrected. Infrared (IR) spectra were recorded on a Bruker alpha FT-IR spectrometer using neat samples.
Commercial HU-331 was purchased from Abcam.
Compound homogeneity was monitored by ascending thin-layer chromatography, performed on Merck 0.2 mm aluminium-backed silica gel 60 F254 plates and visualised using ultraviolet irradiation (254 nm) or by using aqueous alkaline potassium permanganate. Flash column chromatography was performed using Merck silica gel 60 of particle size 33-70 mm. Evaporation refers to the removal of solvent under reduced pressure. Melting points were determined using an Optimet apparatus and are uncorrected. Infrared (IR) spectra were recorded on a Bruker alpha FT-IR spectrometer using neat samples.
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