The gut homogenates were analyzed using BD FACSAria TM Fusion Cell Sorter (Becton Dickinson, Heidelberg, Germany). It utilizes an ion laser emitting a 488 nm wavelength, and a 502 long pass filter, followed by a 530/30 band pass filter. The green fluorescent protein emits light with a peak wavelength of 530 nm. The cells were sorted at a flow rate ranging between 10 µlmin -1 -80 µlmin -1 . The sorting was done in a single-cell mode and the sorted cells were collected in 5 ml sterile Polypropylene round-bottom tubes (Falcon, Mexico). The cells were collected for a period of 3 hours which corresponded to an acquisition of 6000-7000 events/sec.
The flow cytometry grade of PBS buffer (Thermo Fischer, Wilmington, USA) at pH of 7.4 was used as the sheath fluid. A total of ~ 250, 000 cells were sorted from each sample, into 1 ml of RNA Protect solution (Qiagen, Hilden, Germany)
The flow cytometry grade of PBS buffer (Thermo Fischer, Wilmington, USA) at pH of 7.4 was used as the sheath fluid. A total of ~ 250, 000 cells were sorted from each sample, into 1 ml of RNA Protect solution (Qiagen, Hilden, Germany)