Mitochondrial assays were performed in the presence of inhibitors of MAO-A (clorgyline; Cayman Chemical #15925, 2021), MAO-B (rasagiline #SML0124, 2021 and deprenyl #M003,2021; Sigma-Aldrich), or combination MAO-A and MAO-B (pargyline; Sigma-Aldrich #P8013, 2021). The 15.5mM stock compounds were prepared in 100% DMSO and diluted in respiration buffer (215mM mannitol, 75mM sucrose, 0.1% BSA, 20mM HEPES, 2mM MgCl2, and 2.5mM KH2PO4, pH 7.2) so that the concentration of each compound was 10μM after the first port addition (total volume 175μL). For additional bioenergetic assessment of clorgyline, a 20mM stock was made in 100% DMSO and diluted to 10mM in respiration buffer. Serial dilutions were prepared in respiration buffer so that the final concentrations were 5, 10, 20, 40, and 80μM after the first port addition (total volume 175μL). For ROS assessment of clorgyline, 4mM clorgyline stock was prepared in 100% DMSO and diluted in DMSO to 2mM, 1mM, 0.5mM, and 0.25mM to achieve concentrations of 5, 10, 20, 40, and 80μM of clorgyline, respectively, after loading 2μL/dose/well in 100μL total reaction volume. After isolation, mitochondria were in the presence of each treatment for the duration of the following experimental procedures.
Clorgyline
Manufactured by Cayman Chemical
Clorgyline is a laboratory research compound used to selectively inhibit the enzyme monoamine oxidase A (MAO-A). It is commonly utilized in scientific investigations to study the role of MAO-A in various biological processes and pathways.
Automatically generated - may contain errors
Lab products found in correlation
Deprenyl, by Merck Group (1 mentions)
Pargyline, by Merck Group (1 mentions)
Rasagiline, by Merck Group (1 mentions)
Diethylstilbestrol, by Merck Group (1 mentions)
Fluconazole, by Thermo Fisher Scientific (1 mentions)
Toremifene, by Cayman Chemical (1 mentions)
Tamoxifen, by Cayman Chemical (1 mentions)
Raloxifene, by Cayman Chemical (1 mentions)
Glucose, by Merck Group (1 mentions)
Nile red, by Tokyo Chemical Industry (1 mentions)
Itraconazole, by Tokyo Chemical Industry (1 mentions)
Voriconazole, by Tokyo Chemical Industry (1 mentions)
Resveratrol, by Tokyo Chemical Industry (1 mentions)
Gentamicin sulfate, by Chem-Impex (1 mentions)
2 protocols using clorgyline
1
Mitochondrial Assays with MAO Inhibitors
2
Chemical Compounds for Cell Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Clorgyline, clomiphene, toremifene, tamoxifen, raloxifene, and ospemifene were obtained from Cayman Chemicals (Ann Arbor, MI). Fluconazole was purchased from Fisher Scientific (Pittsburgh, PA). Hexestrol, 2-deoxyGlucose, and cis-stilbene were purchased from Alfa Aesar (Tewksbury, MA). Nile red, itraconazole, voriconazole, and resveratrol were purchased from TCI America (Portland, OR). Glucose and diethylstilbestrol were purchased from Sigma-Aldrich (St. Louis, MO). Gentamicin sulfate was obtained from Chem-Impex International Inc. (Wood Dale, IL).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!