Cyclin d1 rabbit polyclonal

As previously described (24 (link), 25 (link)), proteins were extracted and normalized to at least 1500μg/ml. Proteins were subsequently analyzed on SDS-PAGE gels (8% or 10%, as appropriate) and transferred to polyvinylidene difluoride (PVDF) membranes for western blotting (24 (link), 25 (link)).
Primary antibodies that were utilized in this study are: SV40 LTAg mouse monoclonal (#554149, 1:1000, BD Biosciences), DNMT1 rabbit polyclonal (#5032, 1:1000, Cell Signaling Technology), HA-11 mouse monoclonal (#14921901, 1:500, Covance/Lab Corporation), CD71 mouse monoclonal (#sc-51829, 1:250, Santa Cruz Biotechnology), FTH1 rabbit polyclonal (#3998, 1:500, Cell Signaling Technology), pan-Actin rabbit polyclonal (#4968, 1:1000, Cell Signaling Technology), PARP rabbit polyclonal (#9542, 1:1000, Cell Signaling Technology), PAX-8 rabbit polyclonal (#10336-1-AP, 1:1000, Proteintech), Claudin-1 rabbit polyclonal (#187362, 1:10,000, Invitrogen), BAK rabbit monoclonal (#12105, 1: 1000, Cell Signaling Technology), Bcl-2 rabbit monoclonal (#2570, 1:1000, Cell Signaling Technology), Occludin-1 mouse monoclonal (#611090, 1:250, BD Biosciences), Cyclin D1 rabbit polyclonal (#sc-718, 1:1000, Santa Cruz Biotechnology), and Cyclin E mouse monoclonal (#sc-247, 1:500, Santa Cruz Biotechnology).

SDS-PAGE and western analyses were completed as previously described^{89 (link),90 (link)}. Briefly, normalized protein samples were loaded onto either 8% or 10% SDS-PAGE gels (as appropriate) and western blotting was completed with the following antibodies from Cell Signaling Technology (Danvers, MA, USA): (1) EVI1 rabbit monoclonal (#2593, 1:500), (2) Myc rabbit monoclonal (#13987, 1:500), (3) total Ras rabbit monoclonal (#3339, 1:1000), (4) β-catenin rabbit polyclonal (#9587, 1:1000), (5) BMI1 rabbit monoclonal (#6964, 1:1000), (6) LC3B rabbit polyclonal (#2775, 1:1000), and (7) Pan-Actin rabbit polyclonal (#4968, 1:500). SV40 LTAg mouse monoclonal (#554149, 1:1000) and p62 mouse monoclonal (#610832, 1:1000) antibodies were obtained from BD Biosciences (San Jose, CA, USA). FANCD2 mouse monoclonal (#sc-20022, 1:1000), Cyclin D1 rabbit polyclonal (#sc-718, 1:1000), and FoxJ1 mouse monoclonal (#sc-365216, 1:500) were obtained from Santa Cruz Biotechnology.