Paraffin sections of left ventricular myocardium tissues were treated with 3% H2O2 for 10 min, followed by antigen retrieval with citrate buffer. Nonspecific sites were blocked with bovine serum albumin (BSA; Meilunbio, Dalian, China). Sections were incubated with anti-STIM1 (1:100; MA1-19451, Thermo Fisher Scientific, Waltham, MA, USA) and then treated with biotin-conjugated immunoglobulin G (IgG) antibody (1:1,000; PA1-29634, Thermo Fisher Scientific, Waltham, MA, USA). Sections were stained with diaminobenzidine and then counterstained with hematoxylin. The sections were observed under an optical microscope. To evaluate fibrosis, areas of interest (perivascular, and interstitial areas) were defined in Image J. Five fields were randomly selected, and the average value was used as the value of fibrosis area. The extent of fibrosis was determined by calculating the area of perivascular fibrosis, the sum of the interstitial fibrosis areas as a percentage of the total area of the entire section.
Anti stim1
Manufactured by Thermo Fisher Scientific
Sourced in United States
Anti-STIM1 is a laboratory reagent used to detect the presence of STIM1 protein in cell samples. STIM1 is a key regulator of calcium signaling in cells. The anti-STIM1 reagent can be used in various cell biology and biochemistry research applications that require the identification and quantification of STIM1 expression.
Automatically generated - may contain errors
2 protocols using anti stim1
1
Quantifying Myocardial Fibrosis by STIM1 Immunohistochemistry
2
Immunoprecipitation and Immunoblotting of STIM1
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Protein extract was isolated from HL-1 cells using lysis buffer: 1 mg of protein was mixed with 1:1000 anti-STIM1 (Cell Signaling, #4916, Danvers, MA, USA) at 4 °C for 1 h and then incubated with Pierce™ protein A/G magnetic beads (Thermo Fisher Scientific, St. Peters, MO, USA) overnight at 4 °C. The immunoprecipitation matrix was washed twice with PBS containing 1% NP-40. The matrix-bound protein was eluted in sample buffer and then separated by 10% SDS-PAGE electrophoresis. The magnetic beads were saturated to capture all STIM1 proteins in the cell lysate. The immunoblot was done with 1:1000 anti-O-GlcNAc (MA1-076, Thermo Fisher Scientific, St Peters, MO, USA) and 1:1000 anti-STIM1 incubation overnight at 4 °C, and signals were detected by secondary antibodies with chemiluminescent visualization.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!