Anti human cd45 percp cy5.5 hi30

Cryopreserved primary AML cells and engrafted PDX BM cells were thawed and their phenotypes were analyzed using FACSAria2 (BD Biosciences, San Jose, CA, USA) with anti-mouse CD45-APC/Cy7 (30-F11, dilution 1:100), anti-human CD45-PerCP/Cy5.5 (HI30, dilution 1:100) (BioLegend, San Diego, CA, USA), anti-human CD45-PE (HI30, dilution 1:100), anti-human CD34-APC (8G12, dilution 1:100), anti-human CD38-PE/Cy7 (HB7, dilution 1:100), and anti-human CD3-APC (UCHT1, dilution 1:100) antibodies (BD Biosciences, San Jose, CA, USA). Flow cytometry data were collected using FACSAria2 and FACSDiva software v8.0.1 (BD Biosciences, San Jose, CA, USA) and analyzed using FlowJo software v8.8.7 (BD Biosciences, San Jose, CA, USA). The gating strategy is detailed in Supplementary Fig. 6.

anti-human Lineage Cocktail-FITC (CD3, CD14, CD16, CD19, CD20, CD56; UCHT1, HCD14, 3G8, HIB19, 2H7, HCD56; #348801), anti-human CD123-FITC (6H6, #306014), anti-human FcεRIα-FITC (AER-37, #334608), anti-human CD45-PerCP/Cy5.5 (HI30, #304028), anti-human CD117-APC (104D2, #313206), anti-human CRTH2 (CD294)-PE/Cyanine7 (BM16, #350117), anti-human CD127 (IL-7Rα)-Brilliant Violet 421 (A019D5, #351309), anti-human CD183 (CXCR3)-PE (G025H7, #353705), anti-human CD4-PerCP/Cyanine5.5 (OKT4, #317427), anti-human CD196 (CCR6)-PE/Cyanine7 (G034E3, #353417), anti-human CD194 (CCR4)-APC (L291H4, #359407), and anti-human CD45-Brilliant Violet 510 (2D1, #368525) were purchased from BioLegend (San Diego, CA). Anti-CD16/32 antibody was from Miltenyi Biotec (Auburn, CA).