To measure the ABA concentration, the cell-wall mutant and transgenic lines were grown in flasks containing 400 ml TAP medium supplemented with or without 15 nM paraquat. The flasks were incubated at 25 °C with constant shaking (130 rpm) under continuous fluorescent white light (300 µmol m -2 s -1 ). The cells were harvested via centrifugation during exponential growth phase, washed twice with deionized water, and freeze dried using a FreeZone 2.5 Liter lyophilizer (LABCONCO ® , USA). ABA was measured in extracts prepared using 300-500 mg of the lyophilized cells and the ABA ELISA Kit as described by manufacturer's instructions (MyBioSource).
Freezone 2.5 liter
Manufactured by Labconco
Sourced in United States
The FreeZone 2.5 Liter is a laboratory freeze dryer that is designed to concentrate and preserve samples. It has a 2.5 liter collector capacity and can operate at temperatures down to -50°C.
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Lab products found in correlation
2 protocols using freezone 2.5 liter
1
Quantifying Cellular ABA Levels
2
Quantifying Cellular ABA Levels
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To measure the ABA concentration, the cell-wall mutant and transgenic lines were grown in flasks containing 400 ml TAP medium supplemented with or without 15 nM paraquat. The flasks were incubated at 25 °C with constant shaking (130 rpm) under continuous fluorescent white light (300 µmol m -2 s -1 ). The cells were harvested via centrifugation during exponential growth phase, washed twice with deionized water, and freeze dried using a FreeZone 2.5 Liter lyophilizer (LABCONCO ® , USA). ABA was measured in extracts prepared using 300-500 mg of the lyophilized cells and the ABA ELISA Kit as described by manufacturer's instructions (MyBioSource).
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