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Cis 4

Manufactured by Merck Group
Sourced in United States

Cis-4 is a piece of laboratory equipment manufactured by Merck Group. It is designed to perform a specific function within the research and analysis processes. The core function of Cis-4 is to facilitate the separation and isolation of compounds or substances. No further details or interpretation on the intended use of this product can be provided in an unbiased and factual manner.

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3 protocols using cis 4

1

Fatty Acid Analysis Protocol

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The 99%-purity myristic acid (C14:0), palmitic acid (C16:0), palmitoleic acid (C16:1n-9), heptadecanoic acid (C17:0), stearic acid (C18:0), oleic acid (C18:1n-9), linoleic acid (C18:2n-6), linolenic acid (C18:3n-3), Ȗ-linolenic acid (C18:3n-6), cis-11, 14-eicosadienoic acid (C20:2n-6), arachidonic acid (C20:4n-6), cis-5, 8, 11, 14, 17-eicosapentaenoic acid (C20:5n-3), cis-4, 7, 10, 13, 16-docosapentaenoic acid (C22:5n-6), cis-4, 7, 10, 13, 16, 19-docosahexaenoic acid (C22:6n-3), tetracosanoic acid (C24:0), and selacholeic acid (C24:1n-9) from Sigma (St. Louis, MO, USA). H 2 SO 4 (purity: 98.0%), CH 3 OH(GC), n-Hexane (GC), ethyl acetate (AR), NaCl, (AR) Reagent Co. (Tianjin, China).
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2

Lipid Peroxidation Assay Reagents

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Arachidonic acid (AA), cis-4,7,10,13,16,19-docosahexaenoic acid (DHA), cis-5,8,11,14,17-eicosapentaenoic acid (EPA), glycerol (GLY), hexane, phenyl-N-tert-butylnitrone (PBN), propylene glycol (PG), 2,2,6,6-tetramethyl-1-piperidinyloxyl (TEMPO), and tert-benzene, and tris(hydroxymethyl)aminomethane hydrochloride (TRIS-HCl) were purchased from Sigma-Aldrich (St. Louis, MO) and used as received.
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3

DHA and DMSO Immunohistochemistry Protocol

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Cis-4, 7, 10, 13, 16, 19-docosahexaenoic acid (DHA) and dimethyl sulfoxide (DMSO) were from SigmaChemicals (St. Louis, MO). Rat anti-mouse CD16/32 monoclonal antibody was from BD Biosciences (Cat#553141, San Jose, CA). Goat anti-mouse CD206 polyclonal antibody was from R&D Systems (Cat#AF2535, Minneapolis, MN). Rabbit anti-mouse Iba1 polyclonal antibody was from Wako (Cat#019-19741, Richmond, VA). Mouse anti-CHOP monoclonal antibody and rabbit anti-β-actin were from Cell Signaling Technology (Cat#2895, Cat#4970S, Danvers, MA). Rat anti-LAMP-1 monoclonal antibody was from Abcam (Cat#ab25245, Cambridge, MA). Donkey anti-goat Alexa Fluor® 488-conjugated IgG, goat anti-rat Alexa Fluor® 488-conjugated IgG, donkey anti-rabbit Alexa Fluor® 546-cojugated IgG, goat anti-rabbit Alexa Fluor® 546-cojugated IgG, and TO-PRO®-3 iodide were from Invitrogen (Carlsbad, CA). Rabbit anti-nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) p65 was from Santa Cruz Biotechnology (Cat#sc-372, Dallas, TX). Rabbit anti-TATA binding protein was from Abcam (Cat#ab63766, Cambridge, UK). Goat anti-rabbit IgG (H + L)-HRP conjugated was from Bio-Rad (Cat#172-1019, Hercules, CA). Fluoro-Jade C (FJC) was from Histo-Chem Inc. (Jefferson, AK). ELISA kits (DuoSet ELISA) for cytokine measurements were purchased from R&D Systems (Minneapolis, MN, USA).
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