Cultures of the strain ANT-2200 were performed in YPG media at 27 °C in serum bottles (Fig. S1), or in 5 ml syringes immerged in high-pressure incubators as described by Zhang et al. (Zhang, et al., 2016) (link). When indicated, 20 MPa hydrostatic pressure was applied on high pressure incubators that contain the syringes. A stock solution of 20 mM resazurin was prepared in Milli-Q water and used at final concentrations as indicated.
To analyze effect of irradiation, resazurin solutions or bacterial cultures were exposed to tungsten bulb or 470 nm LED. Light densities were measured using Thorlabs S120vc sensor connected to Thorlabs powermeter. Samples were taken at indicated time, and optical density at 600 nm was measured using spectrophotometer and luminescence was analyzed using microplate reader according to fabricant instructions.
To analyze effect of irradiation, resazurin solutions or bacterial cultures were exposed to tungsten bulb or 470 nm LED. Light densities were measured using Thorlabs S120vc sensor connected to Thorlabs powermeter. Samples were taken at indicated time, and optical density at 600 nm was measured using spectrophotometer and luminescence was analyzed using microplate reader according to fabricant instructions.