Ab9324

The antibodies used were as follows: rabbit anti-RhoA (1 : 300, Abcam, ab187027), rabbit anti-ROCK (1 : 200, Abcam, ab125025), rabbit anti-CD 68 (1 : 200, Abcam, ab125212), rabbit anti-CD80 (1 : 200, Abcam, ab215166), rabbit anti-CD163 (1 : 200, Abcam, ab182422), rabbit anti-CD206 (1 : 200, Abcam, ab64693), rabbit anti-inducible nitric oxide synthase (iNOS, 1 : 200, Abcam, ab178945), mouse antitumor necrosis factor-α (TNF-α, 1 : 200, Santa Cruz, sc-52746), mouse anti-interleukin-6 (IL-6, 1 : 300, Abcam, ab9324), and mouse anti-IL-10 (1 : 200, Santa Cruz, sc-365858).

The paraffin-embedded aorta sinus sections (4 μm) were deparaffinized for citrate or EDTA antigen retrieval according to the manufacturer's instructions, blocked with hydrogen peroxide (H₂O₂, 3%), and incubated with different primary antibodies: CD68 (Abcam, ab125212, 1 : 500), TNF-α (Abcam, ab66579, 1 : 200), IL-6 (Abcam, ab9324, 1 : 300), NF-κB (Santa Cruz, sc-8414, 1 : 500), Nrf2 (Abcam, ab137550, 1 : 300), and HO-1 (Abcam, ab13243, 1 : 500) for 60-minute incubation at 37°C. Finally, the sections were washed again with PBS for DAB colorimetry. The positive sections were quantified by Image-Pro Plus 6.0 software (NIH, Bethesda, MD, USA).